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Isolation and Partial Characterization of a 50 kDa Hemin-regulated Cell Envelope Protein from Prevotella nigrescens

Kim KM, Choi JI, Kim SJ

In the study presented here, identification, purification, and partial characterization of a hemin-regulated protein in Prevotella nigrescens were carried out. The results of this study confirm that the availability of...
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Isolation and Partial Characterization of Hemin-binding Cell Envelope Proteins from Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens

Kim SJ

  • KMID: 2462858
  • J Korean Acad Periodontol.
  • 2006 Mar;36(1):155-165.
The results of this study confirm that the availability of hemin influences the expression of selected membrane proteins of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens. A 30 kDa (heated...
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Sequence and phylogenetic analysis of the gp200 protein of Ehrlichia canis from dogs in Taiwan

Huang CC, Hsieh YC, Tsang CL, Chung YT

Ehrlichia (E.) canis is a Gram-negative obligate intracellular bacterium responsible for canine monocytic ehrlichiosis. Currently, the genetic diversity of E. canis strains worldwide is poorly defined. In the present study,...
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The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention

Kim DG, Song CS, Choi IS, Park SY, Lee JB, Lee SS

The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained...
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The centrosomal localization of KM-HN-1 (MGC33607) depends on the leucine zipper motif and the C-terminal coiled-coil domain

Park HJ, Seo HJ, Kim HW, Kim JS, Hwang SY, Seong YS

  • KMID: 1091078
  • Exp Mol Med.
  • 2007 Dec;39(6):828-838.
KM-HN-1 is a C-terminal coiled-coil domain containing protein previously referred to as image clone MGC33607. This protein has been previously identified as a cancer/testis antigen and reported as nuclear and...
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Chestnut as a Food Allergen: Identification of Major Allergens

Lee SK, Yoon SH, Kim SH, Choi JH, Park HS

Chestnut as a Food Allergen: Identification of Major Allergens To evaluate the clinical significance of chestnut as a food allergen in Korea, skin prick test and ELISA were done in...
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Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis

Park WB, Im YB, Shim S, Yoo HS

Brucella abortus is a bacterium that causes brucellosis and is the causative agent of worldwide zoonoses. Pathogenesis of the B. abortus infection is complicated, and several researchers have attempted to...
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Bioassay of human tooth protein blotted polyvinylidene difluoride(PVDF) membrane

Kang N, Hong JR, Choung PH

  • KMID: 1961104
  • J Korean Assoc Oral Maxillofac Surg.
  • 2004 Jun;30(3):186-192.
PURPOSE: Human tooth proteins are highly heterogeneous, comprising diverse proteins derived from a number of genes. The attempts to identify protein for activity of tooth matrix proteins have been defied...
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Substitutions in Penicillin-Binding Protein 1 in Amoxicillin-Resistant Helicobacter pylori Strains Isolated from Korean Patients

Kim BJ, Kim JG

  • KMID: 1712324
  • Gut Liver.
  • 2013 Nov;7(6):655-660.
BACKGROUND/AIMS: A worldwide increase in amoxicillin resistance in Helicobacter pylori is having an adverse effect on eradication therapy. In this study, we investigated the mechanism of the amoxicillin resistance of...
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Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014

Wu Q, Li Z, Zhang G, Niu J, Zeng X, Sun B, Ma J

Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in...
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Expression of neurotensin/neuromedin N precursor in murine mast cells

Ahn HJ, Cho JJ

  • KMID: 1607138
  • Korean J Physiol Pharmacol.
  • 2001 Dec;5(6):495-501.
We have cloned the mouse neurotensin/neuromedin N (NT/N) gene from the murine mast cell line C1.MC/C57.1 for the first time. The murine NT/N cDNA clone consisted of 765 nucleotides and...
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Molecular Characteristics of Extended-Spectrum beta -Lactamases in Clinical Isolates of Enterobacteriaceae

Hong SG, Kang M, Choi JR, Lee K, Chong Y, Kwon OH

  • KMID: 2089686
  • Korean J Clin Pathol.
  • 2001 Dec;21(6):495-504.
BACKGROUND: No significant information on the prevalence of extended-spectrum beta-Lactamase (ESBL)-producing Enterobacteriaceae in Korea has been available, because a few studies conducted to date in Korea have involved only discontinuous...
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Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea

Choi EJ, Lee CH, Song JY, Song HJ, Park CK, Kim B, Shin YK

The high genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) has been an obstacle to developing an effective vaccine for porcine reproductive and respiratory syndrome (PRRS). This study...
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Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

Malik YS, Sharma K, Vaid N, Chakravarti S, Chandrashekar KM, Basera SS, Singh R, Minakshi , Prasad G, Gulati BR, Bhilegaonkar KN, Pandey AB

The present study describes the genotypic distribution of rotaviruses (RVs) in an Indian bovine population with unexpectedly higher proportions of G3 alone or in combination of G8/G10. PCR-genotyping confirmed that...
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Development of a novel vaccine against canine parvovirus infection with a clinical isolate of the type 2b strain

Park SA, Park SY, Song CS, Choi IS, Kim HY, Lee JB, Lee NH

PURPOSE: In spite of an extensive vaccination program, parvoviral infections still pose a major threat to the health of dogs. MATERIALS AND METHODS: We isolated a novel canine parvovirus (CPV) strain...
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Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus

El Hakim A, Shahein YE, Abouelella AM, Selim ME

The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated...
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Isolation and Identification of the Antigen Recognized by Human Cytomegalovirus Specific Monoclonal Antibody SCMVM 34

Park CG, Kook YH, Cha CY, Hwang ES, Lim DG, Seoh JY, Park JW, Jong HS

  • KMID: 1540528
  • J Korean Soc Microbiol.
  • 1997 Jun;32(3):325-334.
Human cytomegalovirus (HCMV)-specific monoclonal antibody, SCMVM 34, recognizes early antigen confined to the nucleus of HCMV-infected cells. This study was performed to identify the antigen reactive to SCMVM 34 with...
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Comparative bioinformatics analysis of prion proteins isolated from reptile, rodent, ruminant, and human species

Ahn I, Son HS

  • KMID: 1091071
  • Exp Mol Med.
  • 2007 Dec;39(6):769-777.
Prion proteins (PrPs) are infectious pathogens that cause a group of invariably fatal, neurodegenerative diseases, including Creutzfeldt-Jakob disease, by means of an entirely novel mechanism. They are produced by various...
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Characterization of a novel anti-human TNF-alpha murine monoclonal antibody with high binding affinity and neutralizing activity

Song MY, Park SK, Kim CS, Yoo TH, Kim B, Kim MS, Kim YS, Kwag WJ, Lee BK, Baek K

In order to develop an anti-human TNF-alpha mAb, mice were immunized with recombinant human TNF-alpha. A murine mAb, TSK114, which showed the highest binding activity for human TNF-alpha was selected...
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Identification of Escherichia coli 8-oxoguanine endonuclease

Lee YS, Chung MH

  • KMID: 755080
  • Exp Mol Med.
  • 2000 Sep;32(3):155-160.
7,8-Dihydro-8-oxoguanine (oh8Gua) endonuclease is a DNA repair enzyme in Escherichia coli to remove oh8Gua, a promutagenic DNA adduct. Due to the unique mode of enzyme action and substrate specificity, this...
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