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Species identification and molecular characterization of Acanthamoeba isolated from contact lens paraphernalia

Lee SM, Choi YJ, Ryu HW, Kong HH, Chung DI

We applied ribosomal RNA gene (rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify Acanthamoeba isolates from contact lens paraphernalia, and characterized these on the basis of mitochondrial DNA...
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Rapid detection of Theileria sergenti by polymerase chain reaction

Choi EJ, Kang SW, Kweon CH, Jeong WS, Yoon YD, Song HJ

Four separate pairs of oligonucleotide primers within the coding region in a T. sergenti 33-kDa surface protein gene were selected to detect T. sergenti by PCR. The specificity of PCR-amplified...
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Detection of Cryptosporidium parvum in Environmental Soil and Vegetables

Hong S, Kim K, Yoon S, Park WY, Sim S, Yu JR

Cryptosporidium parvum is a zoonotic protozoan parasite that causes cryptosporidial enteritis. Numerous outbreaks of cryptosporidiosis have been reported worldwide. Cryptosporidium is transmitted to hosts via consumption of contaminated water and...
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cDNAs encoding the antigenic proteins in pathogenic strain of Entamoeba histolytica

Im KI, Choi JT, Hong YP, Kim TE, La MS

The differential display reverse transcription polymerase chain reaction (DDRT-PCR) analysis was performed to identify the pathogenic strain specific amplicons. mRNAs were purified from the trophozoites of the...
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Detection of Toxoplasma antigens and antibodies in mice infected with different strains of Toxoplasma gondii

Lee YH, Kim KY, Kang MS, Shin DW

This study aims to assess the possible strain-dependent variations in detection of Toxoplasma antigens and antibodies. The virulent RH strain or avirulent Beverley strain of T. gondii were injected into...
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Analysis of antigenic domain of GST fused major surface protein (p30) fragments of Toxoplasma gondii

Nam HW, Im KS, Baek EJ, Choi WY, Cho SY

Antigenic domain of major surface protein (p30) of Toxoplasma gondii was analyzed after polymerase chain reaction (PCR) of its gene fragments. Hydrophilic or hydrophobic moiety of amino acid sequences were...
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Pathogenic free-living amoebae in Korea

Shin HJ, Im KI

Acanthamoeba and Naegleria are widely distributed in fresh water, soil and dust throughout the world, and cause meningoencephalitis or keratoconjunctivitis in humans and other mammals. Korean isolates, namely, Naegleria sp....
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Subgenus classification of Acanthamoeba by riboprinting

Chung DI, Yu HS, Hwang MY, Kim TH, Kim TO, Yun HC, Kong HH

Subgenus classification of Acanthamoeba remains uncertain. Twenty-three reference strains of Acanthamoeba including 18 (neo)type-strains were subjected for classification at the subgenus level by riboprinting. PCR/RFLP analysis of 18S rRNA gene...
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Restriction endonuclease analysis of mitochondrial DNA of Acanthamoeba sp. YM-4 (Korean isolate)

Shin HJ, Im K, Jeon KW

Acanthamoeba sp. YM-4 is similar to A. culbertsoni based upon morphological characteristics of trophozoites and cysts. However, based on other characteristics, pathogenicity to mice, in vitro cytotoxicity and isoenzyme patterns....
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Acanthamoeba sohi, n. sp., a pathogenic Korean isolate YM-4 from a freshwater fish

Im KI, Shin HJ

A new species of Acanthamoeba was isolated from a freshwater fish in Korea and tentatively named Acanthamoeba sp. YM-4 (Korean isolate YM-4). The trophozoites were 11.0-23.0 micrometer in length and...
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Using pig biliary system, in vivo propagation of Enterocytozoon bieneusi, an AIDS-related zoonotic pathogen

Lee JH

  • KMID: 1094648
  • J Vet Sci.
  • 2000 Dec;1(2):105-111.
A microsporidian parasite Enterocytozoon bieneusi is the most common microorganism recognized in AIDS patients, and slow scientific progress is attributed to our inability to propagate the parasite. We report upon...
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Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear

Lee SJ, Jeong HJ, Lee JE, Lee JS, Xuan YH, Kong HH, Chung DI, Ock MS, Yu HS

In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted...
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Delayed Diagnosis of Falciparum Malaria with Acute Kidney Injury

Choi IH, Hwang PH, Choi SI, Lee DY, Kim MS

Prompt malaria diagnosis is crucial so antimalarial drugs and supportive care can then be rapidly initiated. A 15-year-old boy who had traveled to Africa (South Africa, Kenya, and Nigeria between...
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