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An Emerging Diabetes Mellitus Diagnosis Modality: HbA1c

Seo HA, Lee IK

No abstract available.
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Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography

Kong Y, Kang SY, Cho SY

Out of many component proteins in crude saline extract of Spirometra mansoni plerocercoid (sparganum), 36 kDa and 29 kDa proteins were found to be the most antigenic and were already...
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Purification of antigenic protein of sparganum by immunoaffinity chromatography using a monoclonal antibody

Cho SY, Kang SY, Kong Y

The quality improvement of antigen (crude saline extract) of Spirometra mansoni pleroceroid (sparganum) was investigated by protein purification. The crude extract was fractionated by gel filtration through Sephacryl S-300 Superfine....
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Liquid Chromatography-Mass Spectrometry-Based In Vitro Metabolic Profiling Reveals Altered Enzyme Expressions in Eicosanoid Metabolism

Lee SH, Kim EJ, Lee DH, Lee WY, Chung BC, Seo HS, Choi MH

BACKGROUND: Eicosanoids are metabolites of arachidonic acid that are rapidly biosynthesized and degraded during inflammation, and their metabolic changes reveal altered enzyme expression following drug treatment. We developed an eicosanoid...
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Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Measurement of Leukocyte Arylsulfatase A Activity Using a Natural Substrate

Han M, Jun SH, Song SH, Park HD, Park KU, Song J

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Quantitative determination of 12-hydroxyeicosatetraenoic acids by chiral liquid chromatography tandem mass spectrometry in a murine atopic dermatitis model

Hong SH, Han JE, Ko JS, Do SH, Lee EH, Cho MH

Atopic dermatitis, one of the most important skin diseases, is characterized by both skin barrier impairment and immunological abnormalities. Although several studies have demonstrated the significant relationship between atopic dermatitis...
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Quantification of Human Plasma-Busulfan Concentration by Liquid Chromatography-Tandem Mass Spectrometry

Moon SY, Lim MK, Hong S, Jeon Y, Han M, Song SH, Lim KS, Yu KS, Jang IJ, Lee JW, Kang HJ, Song J

BACKGROUND: Busulfan, an alkylating agent administered prior to hematopoietic stem cell transplantation, has a narrow therapeutic range and wide variability in metabolism. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
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Dried Blood Spot Testing for Seven Steroids Using Liquid Chromatography-Tandem Mass Spectrometry With Reference Interval Determination in the Korean Population

Kim B, Lee MN, Park HD, Kim JW, Chang YS, Park WS, Lee SY

BACKGROUND: Conventional screening for congenital adrenal hyperplasia (CAH) using immunoassays generates a large number of false-positive results. A more specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been introduced to...
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A Simple and Rapid Method Based on Liquid Chromatography-Tandem Mass Spectrometry for the Measurement of alpha-L-Iduronidase Activity in Dried Blood Spots: An Application to Mucopolysaccharidosis I (Hurler) Screening

Yang JS, Min HK, Oh HJ, Woo HI, Lee SY, Kim JW, Song J, Park HD

BACKGROUND: We developed an analytical method to measure alpha-L-iduronidase (IDUA) activity in dried blood spots. This was achieved by using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray...
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Evaluation of Serum Cotinine Cut-Off to Distinguish Smokers From Nonsmokers in the Korean Population

Ko K, Kwon MJ, Yang SH, Moon CJ, Lee EH, Woo HY, Park H

BACKGROUND: Cotinine has been widely used as an objective marker to identify current smokers. We conducted this study to address the absence of Korean studies investigating the efficacy of immunoassays...
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Validity of Glycated Hemoglobin in Screening and Diagnosing Type 2 Diabetes Mellitus in Chinese Subjects

Yu Y, Ouyang XJ, Lou QL, Gu LB, Mo YZ, Ko GT, Chow CC, So WY, Ma R, Kong A, Brown N, Nan J, Chan J, Bian RW

BACKGROUND/AIMS: The application of glycated hemoglobin (HbA1c) for the diagnosis of diabetes is currently under extensive discussion. In this study, we explored the validity of using HbA1c as a screening...
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Relationship between Tetrahydrobiopterin and Portal Hypertension in Patients with Chronic Liver Disease

Hong WK, Shim KY, Baik SK, Kim MY, Cho MY, Jang YO, Park YS, Han J, Kim G, Cho YZ, Hwang HW, Lee JH, Chae MH, Kwon SO

Tetrahydrobiopterin (BH4) is an essential cofactor in NO synthesis by endothelial nitric oxide synthase (eNOS) enzymes. It has been previously suggested that reduced intrahepatic BH4 results in a decrease in...
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Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry's HPLC Method

Jiang Y, Liu O, Xu G

BACKGROUND: Reference procedures are required for evaluating the accuracy of routine analytical systems for uric acid (UA). External quality assessment (EQA) for UA has only been conducted with quality controls...
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Development and Evaluation of an Immunochromatographic Kit for the Detection of Antibody to PLASMODIUM VIVAX Infection in South Korea

Park SK, Lee KW, Hong SH, Kim DS, Lee JH, Jeon BH, Kim WS, Shin HJ, An SH, Park H

Malaria is a major parasitic disease in tropical areas. Three to five hundred million people suffer from the disease and it kill a million people per year. Blood smear observation...
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The Combination of Real-Time PCR and HPLC for the Identification of Non-Tuberculous Mycobacteria

Park JS, Choi JI, Lim JH, Ahn JJ, Jegal Y, Seo KW, Ra SW, Jeon JB, Lee SH, Kim SR, Jeong J

We used HPLC and AdvanSure real-time PCR (LG Life Sciences, Korea) to retrospectively analyze non-tuberculous mycobacteria (NTM) in 133 clinical specimens. The specimens were culture-positive for NTM and the HPLC...
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