Yonsei Med J.  1997 Oct;38(5):285-293. 10.3349/ymj.1997.38.5.285.

Granzyme B immunoreactivity in T/natural killer cell lymphomas

Affiliations
  • 1Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
  • 2Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.

Abstract

Granzyme B is one of the serine proteases expressed in natural killer (NK) cells and activated cytotoxic T-lymphocytes. To evaluate the usefulness of granzyme B immunoreactivity in the diagnosis of T/NK-cell lymphoma, we studied 69 cases of lymphomas occurring in the upper aerodigestive tract by paraffin-section immunohistochemistry using a commercially available monoclonal antibody to granzyme B (GrB-7). All 19 cases of T/NK-cell lymphomas defined by the expression of CD56 (NHK-1) and one or both T-cell markers (polyclonal CD3 and CD45RO) expressed granular cytoplasmic granzyme B immunoreactivity. Two out of 9 cases of T-cell lymphomas showing no CD56 expression demonstrated strong granzyme B immunoreactivity. No tumor cells among 39 cases of B-cell diffuse large cell lymphomas and 2 cases of null cell diffuse large cell lymphomas were immunoreactive for granzyme B, however a few scattered granzyme B-positive reactive small lymphoid cells were consistently observed. Based on its sensitivity in this study as well as its reactivity in routinely processed tissue sections, even without heat-induced epitope retrieval technique, monoclonal antibody to granzyme B (GrB-7) can be applied as a useful marker in the diagnosis of T/NK-cell lymphomas in conjunction with CD56.

Keyword

T/NK-cell lymphomas; granzyme B; immunohistochemistry

MeSH Terms

Adult
Aged
Aged, 80 and over
Child
Female
Human
Immunohistochemistry
Killer Cells/pathology*
Lymphoma/pathology*
Lymphoma/enzymology*
Lymphoma, T-Cell/pathology
Lymphoma, T-Cell/enzymology*
Male
Middle Age
Serine Endopeptidases/metabolism*
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