Korean J Parasitol.
1996 Jun;34(2):155-157. 10.3347/kjp.1996.34.2.155.
Survival of Cryptosporidium muris (strain MCR) oocysts under cryopreservation
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- 1Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju, Korea.
- KMID: 658226
- DOI: http://doi.org/10.3347/kjp.1996.34.2.155
Abstract
- We have successfully maintained Cryptosporidium muris by cryopreservation. Oocysts were suspended in distilled water, stored at - 20 degrees C for 24 hrs, and then cryopreserved at -70 degrees C. Cryopreserved specimens were slowly thawed at 5 degrees C. Oocysts, which had been cryopreserved for 15 months without cryoprotective agents, retained their infectivity by the mouse titration method. Oocysts stored at 5 degrees C in 2.5% potassium dichromate failed to retain their infectivity beyond 6.5 months.
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