J Rhinol.  2024 Nov;31(3):133-137. 10.18787/jr.2024.00030.

Human Nasal Turbinate-Derived Stem Cells for Tissue Engineering and Regenerative Medicine

• Kim DH
• Hwang SH

• KMID: 2561494
• DOI: http://doi.org/10.18787/jr.2024.00030

Abstract

Mesenchymal stem cells (MSCs) are multipotent progenitor cells present in adult tissues that are recognized as promising candidates for cell therapy due to their ease of access, straightforward isolation, and capacity for bio-preservation with minimal loss of potency. However, the clinical application of MSCs faces significant challenges, such as donor site morbidity, underscoring the need for alternative sources. Recent studies have suggested that inferior turbinate tissues, which are commonly removed during turbinate surgery, may be a viable donor site for MSCs. Turbinate surgery is a safe and effective procedure frequently performed to alleviate nasal obstruction, a prevalent chronic condition treated by otolaryngologists. This implies that harvesting MSCs from turbinate tissue for tissue engineering and regenerative medicine could serve as a simple, minimally invasive method with faster healing and minimal risk of morbidity or scarring at the donor site. This review highlights previous research indicating that MSCs derived from human turbinate tissues maintain their stability and demonstrate multi-differentiation potential. Therefore, the turbinate could be an alternative to traditional MSC sources for producing functionally competent cells for future clinical applications.

Keyword

Mesenchymal stem cells; Turbinates; Nose; Engineering; Regeneration

Figure

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  Fig. 1. Illustration of the morphology of human nasal turbinate-derived stem cells (hNTSCs) following primary explant culture. The cells adhered to the culture surface and exhibited a spindle-shaped, fibroblast-like morphology, as observed at 100× magnification.

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  Fig. 2. The osteogenic differentiation potential of human nasal turbinate-derived stem cells, as evidenced by alkaline phosphatase staining after 7 and 14 days of osteogenic induction.

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  Fig. 3. The adipogenic differentiation potential of human nasal turbinate-derived stem cells, shown by the presence of intracytoplasmic lipid droplets stained with Oil Red O after 1 and 2 weeks of culture.

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  Fig. 4. The chondrogenic differentiation potential of human nasal turbinate-derived stem cells, with histological analysis showing Alcian blue staining after 1 and 2 weeks of differentiation.

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  Fig. 5. A histological analysis of the respiratory differentiation of human nasal turbinate-derived stem cells, using immunofluorescence staining for cytokeratin-18, cytokeratin-19, MUC2, and mucin-5AC.

Reference

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