J Korean Med Sci.  2024 May;39(17):e157. 10.3346/jkms.2024.39.e157.

Rapid Direct Identification of Microbial Pathogens and Antimicrobial Resistance Genes in Positive Blood Cultures Using a Fully Automated Multiplex PCR Assay

• Kim KJ
• Yun SG
• Cho Y
• Lee CK
• Nam MH

• KMID: 2555498
• DOI: http://doi.org/10.3346/jkms.2024.39.e157

Abstract

This study assessed the performance of the BioFire Blood Culture Identification 2 (BCID2) panel in identifying microorganisms and antimicrobial resistance (AMR) profiles in positive blood cultures (BCs) and its influence on turnaround time (TAT) compared with conventional culture methods. We obtained 117 positive BCs, of these, 102 (87.2%) were correctly identified using BCID2. The discordance was due to off-panel pathogens detected by culture (n = 13), and additional pathogens identified by BCID2 (n = 2). On-panel pathogen concordance between the conventional culture and BCID2 methods was 98.1% (102/104). The conventional method detected 19 carbapenemase-producing organisms, 14 extendedspectrum beta-lactamase-producing Enterobacterales, 18 methicillin-resistant Staphylococcus spp., and four vancomycin-resistant Enterococcus faecium. BCID2 correctly predicted 53 (96.4%) of 55 phenotypic resistance patterns by detecting AMR genes. The TAT for BCID2 was significantly lower than that for the conventional method. BCID2 rapidly identifies pathogens and AMR genes in positive BCs.

Keyword

Blood Culture; Bloodstream Infection; Rapid Diagnostics; Molecular Detection; Sepsis; Species Identification; Antimicrobial Susceptibility Testing

Figure

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  Fig. 1 A flow chart showing the performance of the BCID2 identification results compared to the conventional MALDI-TOF MS identification.BCID2 = BioFire Blood Culture Identification 2, MALDI-TOF MS = matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.aMonomicrobial or polymicrobial identification was classified according to the conventional MALDI-TOF MS results.

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