Int Neurourol J.
2020 Nov;24(Suppl 2):79-87. 10.5213/inj.2040428.214.
Polydeoxyribonucleotide Ameliorates Inflammation and Apoptosis in Achilles Tendon-Injury Rats
- Affiliations
-
- 1Department of Anesthesiology and Pain Medicine, Okcheon St. Mary’s Hospital, Okcheon, Korea
- 2Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea
- 3Department of Sport & Health Care, College of Art & Culture, Sangmyung University, Seoul, Korea
- 4Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea
- 5Department of Medicine, Graduate School, Kyung Hee University, Seoul, Korea
- 6Department of Anesthesiology and Pain Medicine, Kyung Hee University Medical Center, College of Medicine, Kyung Hee University, Seoul, Korea
- KMID: 2510832
- DOI: http://doi.org/10.5213/inj.2040428.214
Abstract
- Purpose
Adenosine A2A receptor agonist polydeoxyribonucleotide (PDRN) possesses an anti-inflammatory effect and suppress apoptotic cell death in several disorders. In this current study, the effect of PDRN on inflammation and apoptosis in rats with Achilles tendon injury was investigated.
Methods
von Frey filament test and plantar test were conducted for the determination of pain threshold. Analysis of histological alterations was conducted by hematoxylin and eosin staining. Immunohistochemistry for cleaved caspase-3-positive cells and cleaved caspase-9-positive cells was done. Enzyme-linked immunoassay was used to detect the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and cyclic adenosine-3’,5’-monophosphate (cAMP). Western blot was conducted to detect the protein levels of cAMP response element-binding protein (CREB), protein kinase A (PKA), Bcl-2-associated X (Bax), and B-cell lymphoma 2 (Bcl-2).
Results
PDRN treatment relieved mechanical allodynia and alleviated thermal hyperalgesia after Achilles tendon injury. TNF-α and IL-6 concentrations were decreased by PDRN application. PDRN injection significantly enhanced cAMP concentration and phosphorylated CREB versus CREB ratio, showing cAMP-PKA-CREB pathway was activated by PDRN application. PDRN treatment inhibited percentages of cleaved caspase-3-positive cells and caspase-9-posiive cells and the suppressed Bax versus Bcl-2 ratio in Achilles tendon injury rats.
Conclusions
PDRN is probably believed to have a good effect on pain and inflammation in the urogenital organs. PDRN may be used as a new treatment for Achilles tendon injury.
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