J Korean Acad Oral Health.  2020 Jun;44(2):73-77. 10.11149/jkaoh.2020.44.2.73.

Determination of the infection rate of dental caries-causing bacteria in adolescents by a real-time PCR test method

Affiliations
  • 1Department of Oral Health, Graduate School of Public Health and Social Welfare, Dankook University, Cheonan,
  • 2Department of Dental Hygiene, Gangneung Yeongdong University, Gangneung,
  • 3Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Korea

Abstract


Objectives
The aim of this study was to investigate the infection rates of bacteria associated with dental caries among children and adolescents in Korea.
Methods
Oral examinations were conducted in 146 students. The numbers of bacteria associated with dental caries, such as Streptococcus mitis , Streptococcus mutans , Streptococcus sobrinus , and Lactobacillus casei , in the subjects’ oral cavity were counted by real-time PCR, and infection rates for those bacteria were additionally investigated.
Results
The infection rate of Streptococcus mitis , Streptococcus mutans , Streptococcus sobrinus , and Lactobacillus casei was 99.3% (145/146), 63.0% (92/146), 1.4% (2/146), and 25.3% (37/146), respectively. The amounts of Streptococcus sobrinus and Lactobacillus casei did not differ with the participants’ age. However, the number of Streptococcus mutans was 537.6 for primary school students, 5698.2 for middle school students, and 19037.5 for high school students. The mean number of oral bacteria increased with age (P<0.05). The mean bacterial numbers of the infected subjects indicated significant differences in the numbers of Streptococcus mitis and Streptococcus mutans (P<0.05).
Conclusions
The infection rates of Streptococcus mitis and Streptococcus mutans were distinct in children and adolescents. Efforts to control the bacteria associated with dental caries are needed to prevent dental caries.

Keyword

Infection rate; Oral microorganism; Streptococcus mutans
Full Text Links
  • JKAOH
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2020 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr