J Korean Acad Oral Health.  2020 Sep;44(3):126-129. 10.11149/jkaoh.2020.44.3.126.

Caries activity test by realtime PCR method and bacterial cultural method

• Yun JH
• Park JH
• Cho JW
• Kim SW

• KMID: 2506659
• DOI: http://doi.org/10.11149/jkaoh.2020.44.3.126

Abstract

Objectives
This study aimed to compare the results of conventional methodology and real-time polymerase chain reaction (PCR) for acid-producing bacteria and identify the appropriate method for caries activity testing.
Methods
Oral examination and caries activity tests, such as the CRT^® Bacteria test and modified Snyder test, were conducted on 33 middle school students in Cheongju, South Korea. Pearson correlation analysis was subsequently performed with a significance level of 5%.
Results
The amount of Streptococcus mutans determined using the CRT^® Bacteria test was highly correlated with the decayed teeth (DT) index (R=0.570, P<0.05); decayed, missing, and filled teeth (DMFT) index (R=0.376, P<0.05); and dental health capacity of the first permanent molar (R=―0.395, P<0.05). The amount of Streptococcus mutans determined using real-time PCR was significantly associated with the DT index (R=0.528, P<0.05), DMFT index (R=0.369, P<0.05), and dental health capacity of the first permanent molar (R=―0.426, P=0.013).
Conclusions
There was a significant correlation between the amount and the type of bacteria between the CRT^® Bacteria test and real-time PCR; therefore, these tests were found to be more appropriate to determine caries activity than the modified Snyder test. This study suggests that realtime PCR is a better technique for detecting caries activities than conventional techniques, such as the CRT^® Bacteria test and modified Snyder test, because it is easy to use and provides accurate results.

Keyword

Caries activity test; Oral microorganism; Streptococcus mutans

Figure

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  Fig. 1 CRT® bacteria score reading.

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  Fig. 2 Standardization of color changes for modifided snyder test.

Reference

1. Paik DI, Kim HD, Shin SC, Cho JW, Park YD, Kim DK, et al. 2011. Clinical preventive dentistry. 5th ed. Komoonsa;Seoul: p. 23–41. p. 265–287.

2. Ali YA, Chandranee NJ, Wadher BJ, Khan A, Khan ZH. 1998; Relationship between caries status, colony forming units (cfu) of Streptococcus mutans and Snyder caries activity test. J Indian Soc Pedod Prev Dent. 16:56–60. PMID: 11813756.

3. Ramesh K, Kunjappan S, Ramesh M, Shankar S, Reddy S. 2013; Comparative evaluation of predictive value of three caries activity tests-snyder, lactobacillus count and cariostat in mixed dentition children with and without caries. J Pharm Bioallied Sci. 5(Suppl 1):S63–68. DOI: 10.4103/0975-7406.113299. PMID: 23946580. PMCID: PMC3722709.

4. Sims W. 1968; A modified Snyder test for caries-activity in humans. Arch Oral Biol. 13:853–856. DOI: 10.1016/0003-9969(68)90001-0. PMID: 3503962.

5. Darwita RR, Andreas P. 2013; Salivary parameters of buffer capacity, pH saliva and pH plaque related to dental caries activity in school student. Int J Clin Prev Dent. 9:145–148. DOI: 10.1111/j.1741-2358.1999.00047.x. PMID: 10687508.

6. Kim JG, Kim YS, Baik BJ, Yang YM. 2005; Relationship between salivary caries-related tests and dental caries experience in Korean dental college students. J Korean Acad Pediatr Dent. 32:67–74.

7. Jeon YM, Moon KH, Cho JW. 2015; Prevalence of oral microbes known to be the cause of in patients of 20s with early gingivitis. Int J Clin Prev Dent. 11:97–100. DOI: 10.15236/ijcpd.2015.11.2.97.

8. Jeon YM, Cho JW, Lee JY, Woo SH. 2015; The effects of oral microorganism on oral malodor. Int J Clin Prev Dent. 11:171–176. DOI: 10.15236/ijcpd.2015.11.3.171. PMID: 11944909.

9. Kim JB, Choi EG, Moon HS, Kim JB, Kim DK, Lee HS, et al. 2004. Public health dentistry. 4th ed. Komoonsa;Seoul: p. 303–378.

10. Lee JW, Kim MS, Choi YH, Chang YS, Shin SC. 2008; A study on the relation of the stimulated salivary flow rate, pH and the viscosity. Int J Clin Prev Dent. 4:112–122.

11. Kim JB, Choi EG, Paik DI, Shin SC, Chang KW, Hong SJ, et al. 2008. Preventive dentistry. 5th ed. Komoonsa;Seoul: p. 301–318.

12. Shin DK, Kim JY, Song KB, Nam SH. 2003; Relationship between Dentocult-SM test, microbial analysis and dental caries in the pre-school children. J Korean Acad Pediatr Dent. 30:254–262. DOI: 10.17219/acem/28998. PMID: 26935493.

13. Chang YS, Jung MA, Shin SC. 2008; Evaluation of motility and distribution of oral micro-flora in Korean using the phase contrast microscope. Int J Clin Prev Dent. 4:28–39.

14. Jung KY, Chang YS, Lee YJ, Woo SH. 2008; Correlation coefficient between the amount and the motile of various oral microorganism. Int J Clin Prev Dent. 4:168–176. DOI: 10.1111/andr.12595. PMID: 30786164. PMCID: PMC6767197.

15. Kim DH, Park JH, Yun MH, Shin SC, Cho JW. 2014; The differential weight of factors for estimation of individual oral health index. Int J Clin Prev Dent. 10:37–43. DOI: 10.15236/ijcpd.2014.10.1.37. PMID: 31499737. PMCID: PMC6765335.