J Rhinol.  2020 May;27(1):8-15. 10.18787/jr.2018.00277.

Polysaccharide from Hizikia Fusiformis Enhances the Immunomodulatory Activity of Macrophages

  • 1Department of Otorhinolaryngology Head & Neck Surgery, Medical College of Chosun University, Gwangju, Korea
  • 2Department of Biochemical and Polymer Engineering, Chosun University, Gwangju, Korea


Background and Objectives
Hizikia fusiformis is widely used in oriental health food in Japan, China, and Korea, and is known for its anti-oxidation properties. Materials and Method: In this study, we investigated the anti-inflammatory and immune-modulatory effects and mechanisms of Hizikia fusiformis (H. fusiformis) extracts in lipopolysaccharide (LPS)-treated RAW 264.7 cells. RAW 264.7 cells were incubated in the presence of different concentrations of the viscozyme component of H. fusiformis (1, 2, 5, and 10 μg/mL), and changes in expression of pro-inflammatory cytokines (GM-CSF, iNOS, VEGF, and COX-2) were evaluated by real-time PCR and immunoblotting. In addition, the associated signaling pathway including phospho (p)-pNF-κB 65, p-pIkBa, p-p38, and p-p44/42 was also evaluated.
The viscozyme component of H. fusiformis downregulated the expression of GM-CSF, iNOS, VEGF, and COX-2 mRNA. The augmented NO and ROS production was decreased by administration of H. fusiformis. The signal intensity of p-pNF-κB 65, p-pIkBa, p-p38, and p-p44/42 protein activated by LPS was ameliorated by administration of the viscozyme fraction in RAW 264.7 cells.
These results suggest that H. fusiformis has potential as a therapeutic agent for inflammatory diseases.


Sargassum; Polysaccharides; NF-kappa B; Inflammation; Immunity
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