Korean J Parasitol.  2020 Apr;58(2):161-171. 10.3347/kjp.2020.58.2.161.

Changes in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis

Affiliations
  • 1Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024, PR China
  • 2Instrumental Analysis Center, Hebei Normal University, Shijiazhuang, Hebei 050024, PR China
  • 3Department of Pathogenic Biology, College of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China
  • 4State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, PR China

Abstract

The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.

Keyword

Tick; salivary gland; apoptosis; data-independent acquisition; RNA interference; Hippo
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