J Cancer Prev.  2019 Dec;24(4):240-244. 10.15430/JCP.2019.24.4.240.

Usefulness Analysis of Urine Samples for Early Screening of Human Papilloma Virus Infection

• Choi YS
• Jin H
• Lee KE

• KMID: 2468814
• DOI: http://doi.org/10.15430/JCP.2019.24.4.240

Abstract

Human papilloma virus (HPV) is known to be a major cause of cervical cancer. In Korea, although the mortality of cervical cancer has decreased, HPV infection rates are increasing rapidly in young women. One of the reasons for a high rate of human immunodeficiency virus (HIV) infection appears to be associated with a low frequency to visit gynecology clinics because of the uncomfortable sampling process for HPV testing. Therefore, it is necessary to develop a non-invasive method, such as urine testing to diagnose cervical cancer rather than use of the existing invasive method. This study aimed to test validity of HPV DNA detection in urine specimens that can be easily collected from women. Paired vaginal discharge and urine samples were collected prospectively from 203 women who visited the local hospital between January and August 2018 in Busan, Korea. By using the Virocheck® assay kit (Optipharm), we found that 17.2% (35/203) of vaginal discharge samples were HPV positive and 82.8% (168/203) were HPV negative. In urine samples, 15.8% (32/203) were HPV positive and 84.2% (171/203) were HPV negative. The co-incident rate for HPV DNA detection was 84.8% in both vaginal discharge and urine samples. These results suggest that the HPV DNA detection using urine samples might be an alternative way to diagnose HPV infection in a non-invasive way. This analytical approach can be utilized as a screening test to identify HIV-infected patients who need a follow-up process by using urine samples.

Keyword

Human papilloma virus; Vaginal discharge; Urine

MeSH Terms

Busan
DNA
Female
Follow-Up Studies
Gynecology
HIV
Humans*
Korea
Mass Screening*
Methods
Mortality
Papillomaviridae*
Prospective Studies
Uterine Cervical Neoplasms
Vaginal Discharge
DNA