Nat Prod Sci.  2019 Jun;25(2):150-156. 10.20307/nps.2019.25.2.150.

Simultaneous Extraction and Separation of Oil and Azadirachtin from Seeds and Leaves of Azadirachta indica using Binary Solvent Extraction

• Subramanian S
• Salleh AS
• Bachmann R
• Hossain MS

• KMID: 2452785
• DOI: http://doi.org/10.20307/nps.2019.25.2.150

Abstract

Conventional extraction of oil and azadirachtin, a botanical insecticide, from Azadirachta indica involves defatting the seeds and leaves using hexane followed by azadirachtin extraction with a polar solvent. In order to simplify the process while maintaining the yield we explored a binary extraction approach using Soxhlet extraction device and hexane and ethanol as non-polar and polar solvents at various ratios and extraction times. The highest oil and azadirachtin yields were obtained at 6 h extraction time using a 50:50 solvent mixture for both neem leaves (44.7 wt%, 720 mg(Aza)/kg(leaves)) and seeds (53.5 wt%, 1045 mg(Aza)/kg(seeds)), respectively.

Keyword

Azadirachtin; limonoids; neem oil; binary solvent extraction; botanical insecticide

MeSH Terms

Azadirachta*
Ethanol
Limonins
Solvents
Ethanol
Limonins
Solvents

Figure

• 

  Fig. 1 Neem oil yields (a) and azadirachtin yields (b) from neem leaves at various solvent ratios and extraction times (n=3).

• 

  Fig. 2 Neem oil yield (a) and azadirachtin yields (b) from neem seeds at various solvent ratios and extraction times (n=3).

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