Nat Prod Sci.  2023 Jun;29(2):83-90. 10.20307/nps.2023.29.2.83.

Optimization of the Extraction of Polyphenols and Flavonoids from Argania spinosa Leaves using Response Surface Methodology

  • 1Microbiology, Health and Environment Team, Faculty of Sciences, Chouaïb Doukkali University, El Jadida 24000, Morocco
  • 2Health and Environment Laboratory, Ain Chock Faculty of Sciences, Hassan II University of Casablanca 20000, Morocco


To our knowledge, this is the first study aiming to optimize the extraction conditions of total phenolic compounds (TPC) and total flavonoids contents (TFC) from Argania spinosa leaves using Response Surface Methodology (RSM) with a Box–Behnken design (BBD). The optimal conditions obtained were 5% (w/v) solvent-to-solid ratio, 72.33% ethanol concentration, and 10h ours as an extraction time, which resulted in an extract with maximum TPC (131.63 mg GAE/g dw) and TFC (10.66 mg QE/g dw). Under the optimal extraction conditions, the antioxidant activity of the extracts of leaves of argan tree showed a moderate antiradical capacity of DPPH (IC 50 = 0,130 mg/mL) and ABTS (IC 50 = 0.198 mg/mL). However, the leaves of argan tree showed a very interesting reducing power of Iron (IC 50 = 0.448 mg/ml) which is similar to that of the ascorbic acid (IC 50 = 0.371 mg/mL).


Argania spinosa Leaves; optimization; response surface methodology; phenolic compounds; antioxidant activities
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