Nat Prod Sci.  2018 Dec;24(4):241-246. 10.20307/nps.2018.24.4.241.

Identification and HPLC Quantification of a Phytoecdysone and Three Phenolic Glycosides in Lamium takesimense Nakai

Affiliations
  • 1Department of Agro-industrial Technology, LambungMangkurat University, Banjarbaru 70714, Indonesia.
  • 2Deparment of Oriental Medicine, Sangji University, Wonju 26339, Korea.
  • 3Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea. hjpark@sangji.ac.kr

Abstract

The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 µm, 4.6 mm × 250 mm) with a gradient elution of Hâ‚‚O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order: lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g).

Keyword

Lamium takesimense Nakai; Lamiaceae; ecdysone; rutin; HPLC

MeSH Terms

Acetic Acid
Chromatography, High Pressure Liquid*
Ecdysone
Ecdysterone
Glycosides*
Lamiaceae
Methods
Phenol*
Plants
Rutin
Acetic Acid
Ecdysone
Ecdysterone
Glycosides
Phenol
Rutin
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