Nat Prod Sci.  2018 Dec;24(4):241-246. 10.20307/nps.2018.24.4.241.

Identification and HPLC Quantification of a Phytoecdysone and Three Phenolic Glycosides in Lamium takesimense Nakai

• Nugroho A
• Park KS
• Seo DJ
• Park HJ

• KMID: 2432418
• DOI: http://doi.org/10.20307/nps.2018.24.4.241

Abstract

The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 µm, 4.6 mm × 250 mm) with a gradient elution of Hâ‚‚O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order: lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g).

Keyword

Lamium takesimense Nakai; Lamiaceae; ecdysone; rutin; HPLC

MeSH Terms

Acetic Acid
Chromatography, High Pressure Liquid*
Ecdysone
Ecdysterone
Glycosides*
Lamiaceae
Methods
Phenol*
Plants
Rutin
Acetic Acid
Ecdysone
Ecdysterone
Glycosides
Phenol
Rutin

Figure

• 

  Fig. 1 Structure of compounds 1 – 4 isolated from L. takesimense.

• 

  Fig. 2 HPLC chromatograms of the MeOH extract of L. takesimese and its CHCl3 and BuOH fractions.

Reference

1. Dinan L. Phytochemistry. 2014; 57:325–339.

2. Wang T, Zhang X, Xie W. Am J Chin Med. 2012; 40:1123–1141.

3. Kurisu M, Miyamae Y, Murakami K, Han J, Isoda H, Irie K, Shigemori H. Biosci Biotechnol Biochem. 2013; 77:1329–1332.

4. Lee CB. Colored Flora of Korea. Seoul: Hyangmunsa;2014. p. 129.

5. Ito N, Nihei T, Kakuda R, Yaoita Y, Kikuchi M. Chem Pharm Bull. 2006; 54:1705–1708.

6. Agzmova MA, Isaev IMO, Mamathanov AU, Isaev MIO, Ibragimov TF. Adv Biol Chem. 2014; 4:1–4.

7. Schlauer J, Budzianowski J, Kukulczanka K, Ratajczak L. Acta Soc Bot Pol. 2004; 73:9–15.

8. Park HW, Baek NI, Kim SH, Kwon BM, Chung IS, Park MH, Kim SH, Kim DK. Korean J Pharmacogn. 2004; 35:320–323.

9. Báthori M, Tóth N, Hunyadi A, Márki A, Zádor E. Curr Med Chem. 2008; 15:75–91.