Korean J Phys Anthropol.  2018 Dec;31(4):133-142. 10.11637/kjpa.2018.31.4.133.

3D Histology Using the Synchrotron Radiation Propagation Phase Contrast Cryo-microCT

Affiliations
  • 1Department of Anatomy, Catholic University of Daegu School of Medicine, Korea. htaekim@cu.ac.kr
  • 2Department of Radiology, Yeongju Red Cross Hospital, Korea.
  • 3Optical Convergence Technology Center, Catholic University of Daegu, Korea.
  • 4Department of Parasitology, Catholic University of Daegu School of Medicine, Korea.
  • 5Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, Korea.

Abstract

3D histology is a imaging system for the 3D structural information of cells or tissues. The synchrotron radiation propagation phase contrast micro-CT has been used in 3D imaging methods. However, the simple phase contrast micro-CT did not give sufficient micro-structural information when the specimen contains soft elements, as is the case with many biomedical tissue samples. The purpose of this study is to develop a new technique to enhance the phase contrast effect for soft tissue imaging. Experiments were performed at the imaging beam lines of Pohang Accelerator Laboratory (PAL). The biomedical tissue samples under frozen state was mounted on a computer-controlled precision stage and rotated in 0.18° increments through 180°. An X-ray shadow of a specimen was converted into a visual image on the surface of a CdWO4 scintillator that was magnified using a microscopic objective lens (X5 or X20) before being captured with a digital CCD camera. 3-dimensional volume images of the specimen were obtained by applying a filtered back-projection algorithm to the projection images using a software package OCTOPUS. Surface reconstruction and volume segmentation and rendering were performed were performed using Amira software. In this study, We found that synchrotron phase contrast imaging of frozen tissue samples has higher contrast power for soft tissue than that of non-frozen samples. In conclusion, synchrotron radiation propagation phase contrast cryo-microCT imaging offers a promising tool for non-destructive high resolution 3D histology.

Keyword

3D histology; Synchrotron radiation; Cryo-microtomography; X-ray propagation phase contrast; Collagen induced arthritis
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