Yonsei Med J.  2009 Jun;50(3):422-426. 10.3349/ymj.2009.50.3.422.

Phase Contrast Microradiography of Mouse Lung Using Synchrotron X-ray: Correlation with Optical Microscopy

Affiliations
  • 1Department of Radiology, Korea University Guro Hospital, Seoul, Korea. keyrad@korea.ac.kr
  • 2Department of Pathology, Korea University Guro Hospital, Seoul, Korea.
  • 3The Research Institute for Skin Imaging, Korea University Guro Hospital, Seoul, Korea.
  • 4Department of Material Science and Engineering, Pohang University of Science and Technology, Pohang, Korea.
  • 5Department of Radiology, Kangwon National University College of Medicine, Chuncheon, Korea.
  • 6Department of Radiology, Chung-Ang University College of Medicine, Seoul, Korea.

Abstract

PURPOSE: The purpose of this study is to evaluate the feasibility of phase contrast X-ray microtomography and microradiography, using a polychromatic synchrotron X-ray, for analysis of the mouse lung microstructure.
MATERIALS AND METHODS
Normal mice were used for experiments. Some of the mouse lungs were prepared by the lung fixation-inflation method. The resulting sponge-like inflated lung samples were used for microtomography. The remaining mouse lungs were cut into 10 um sections and were used for microradiography and optical microscopic correlation. The experiments on mouse lung samples were performed at the 7B2 beamline of the Pohang Light Source in Korea.
RESULTS
Phase contrast X-ray microtomography of inflated lung samples showed individual alveolar structure on 3-D reconstruction. Phase contrast microradiographs of thin lung samples showed microstructure of lung, such as alveoli and bronchioles, and were well correlated with optical microscopic images.
CONCLUSIONS
The results indicate that the phase contrast X-ray microtomography and microradiography using polychromatic synchrotron X-ray is feasible for evaluation of microstructure of the lung.

Keyword

Phase contrast microradiography; polychromatic synchrotron X-ray; refraction enhanced imaging; lung

MeSH Terms

Animals
Lung/*cytology/*radiography
Mice
Microscopy/*methods
Microscopy, Phase-Contrast
X-Ray Microtomography/*methods
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