Int Neurourol J.  2016 Jun;20(2):122-130. 10.5213/inj.1632552.276.

Increased Expression of Herpes Virus-Encoded hsv1-miR-H18 and hsv2-miR-H9-5p in Cancer-Containing Prostate Tissue Compared to That in Benign Prostate Hyperplasia Tissue

  • 1Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea.
  • 2Department of Anesthesiology and Pain Medicine, Inha University College of Medicine, Incheon, Korea.
  • 3Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea.
  • 4Bio Medical Laboratories, Daejeon, Korea.
  • 5Department of Surgery, Chungbuk National University, College of Medicine and Medical Research Institute, Cheongju, Korea.
  • 6Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea.
  • 7NAR Center Inc., Daejeon Oriental Hospital of Daejeon University, Daejeon, Korea.
  • 8The Laboratory of Vascular Biology and Stem Cells, BioMedical Research Center, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea.
  • 9Medical Genomics Research Center, Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Department of Functional Genomics, University of Science and Technology, Daejeon, Korea.
  • 10Biomedical Research Institute, Department of Neuropsychiatry, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seoul, Korea.
  • 11Department of Pathology, Chungbuk National University College of Medicine, Cheongju, Korea.
  • 12School of Food Science and Technology, Chung-Ang University, Anseong, Korea.
  • 13Department of Animal Sciences, Chungbuk National University, Cheongju, Korea.
  • 14The Section of Urologic Oncology and Dean and Betty Gallo Prostate Cancer Center, The Cancer Institute of New Jersey, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, NJ, USA.
  • 15Department of Surgery, Harvard Medical School, Boston, MA, USA.
  • 16Division of Cancer Biology and Therapeutics, Departments of Surgery and Biomedical Sciences, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
  • 17Department of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
  • 18Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, Korea.
  • 19Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan, Korea.
  • 20Department of Biomedical Engineering, Chungbuk National University, Cheongju, Korea.


Previously, we reported the presence of virus-encoded microRNAs (miRNAs) in the urine of prostate cancer (CaP) patients. In this study, we investigated the expression of two herpes virus-encoded miRNAs in prostate tissue.
A total of 175 tissue samples from noncancerous benign prostatic hyperplasia (BPH), 248 tissue samples from patients with CaP and BPH, and 50 samples from noncancerous surrounding tissues from these same patients were analyzed for the expression of two herpes virus-encoded miRNAs by real-time polymerase chain reaction (PCR) and immunocytochemistry using nanoparticles as molecular beacons.
Real-time reverse transcription-PCR results revealed significantly higher expression of hsv1-miR-H18 and hsv2-miRH9- 5p in surrounding noncancerous and CaP tissues than that in BPH tissue (each comparison, P<0.001). Of note, these miRNA were expressed equivalently in the CaP tissues and surrounding noncancerous tissues. Moreover, immunocytochemistry clearly demonstrated a significant enrichment of both hsv1-miR-H18 and hsv2-miR-H9 beacon-labeled cells in CaP and surrounding noncancerous tissue compared to that in BPH tissue (each comparison, P<0.05 for hsv1-miR-H18 and hsv2- miR-H9).
These results suggest that increased expression of hsv1-miR-H18 and hsv2-miR-H95p might be associated with tumorigenesis in the prostate. Further studies will be required to elucidate the role of these miRNAs with respect to CaP and herpes viral infections.


MicroRNAs; Prostate Neoplasms; Herpesviridae; Prostate Hyperplasia
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