J Korean Burn Soc.  2016 Dec;19(2):82-87. 10.0000/jkbs.2016.19.2.82.

Development and Evaluation of Acellular Dermal Matrix Originated from Transgenic Porcine Skin for Primates Skin Graft

• Lee KW
• Choi GS
• Kim MJ
• Lee JS
• Yoon H
• Chun W

• KMID: 2361631
• DOI: http://doi.org/10.0000/jkbs.2016.19.2.82

Abstract

This study was performed to compare the healing quality of the allogenic acellular dermal matix (ADM) and xenogenic ADM combined with autologous split thicknessskin graft. Xenogenic ADM was obtained from two wild type pigs. Allogenic ADM was obtained from cynomolgus monkeys. ADM was stored with cryo-preservation. Full-thickness skin wounds were made on the back of two cynomolgus monkeys. In one monkey, wounds were covered by allogenic ADM combined with autologous split thickness skin graft (STSG) or autologous STSGonly. In another monkey, wounds were covered by xenogenic ADM combined with autologous skin graft or autologous skin graft only. Skin healing process was observed during 2 weeks and skin biopsies were performed on 3 months after skin transplantation. We obtained IACUC approval (ORIENT-IACUC-16053). Skin on the xenogenic ADM was necrotized 1 week after skin transplantation. Possibly due to the thickness of ADM, which block the blood supply from the subcutaneous tissue to the autologous skin graft. Skin biopsy revealed that less fibrotic change of the skin on the ADM compared with the skin without ADM. Xenogenic ADM can be used in high degree burn patients who can suffered from contracture after healing since it can reduce fibrotic change.

Keyword

Transgenic pig; Acellular dermal matrix; Xeno-skin transplantation

MeSH Terms

Acellular Dermis*
Animal Care Committees
Biopsy
Burns
Contracture
Haplorhini
Humans
Macaca fascicularis
Primates*
Skin Transplantation
Skin*
Subcutaneous Tissue
Swine
Transplants*
Wounds and Injuries