J Korean Pediatr Soc.  2001 Apr;44(4):397-404.

The Effect of rhG-CSF and rhGM-CSF on Expression of Fc gamma Receptors and beta2 Integrin and Respiratory Burst Function in Human Granulocytes

• Kim KH
• Ryu KH
• Seoh JY

Abstract

PURPOSE
Receptors for the Fc of IgG(FcvR) and a beta2 integrin molecule, CD11c/CD18 are important in clearance of microbes by granulocytes. We performed an in vitro study on the effect of recombinant human granulocyte colony-stimulating factor(rhG-CSF), or granulocyte-macrophage colony-stimulating factor(rhGM-CSF) on the expression of Fc R II, Fc R III, CD11c and CD18 and respiratory burst function of granulocytes.
METHODS
Peripheral blood was collected from six healthy adults. The isolated granulocytes were stimulated with rhG-CSF, 250mg/mL, rhGM-CSF, 100ng/mL or both of them for 1, 3, 9, 24, and 48 hours. Using flow cytometry, we compared the expression of the Fc R II, Fc R III, CD11c and CD18 of granulocytes before and after stimulation. We also the studied respiratory burst of granulocytes with chemiluminescence assay.
RESULTS
Fc R II and CD18 expression were not changed significantly after stimulation. Fc R III expression was decreased significantly after stimulation with rhG-CSF, rhGM-CSF, or both of them. CD11c expression was increased initially but was found to decrease significantly after 9 hours of stimulation. Granulocytes treated with rhG-CSF, rhGM-CSF, or both displayed enhanced respiratory burst. Our results suggest that exposure of granulocyte to growth factor results in granulocyte activation.
CONCLUSION
We have shown that rhG-CSF and rhGM-CSF resulted in an activation of peripheral blood granulocytes immunophenotypically and functionally.

Keyword

Granulocytes; rhG-CSF; rhGM-CSF; FcvR; beta2 Integrin

MeSH Terms

Adult
Antigens, CD18*
Flow Cytometry
Granulocytes*
Humans*
Luminescence
Receptors, IgG*
Respiratory Burst*
Antigens, CD18
Receptors, IgG