Nat Prod Sci.  2016 Jun;22(2):111-116. 10.20307/nps.2016.22.2.111.

Chemical Components from the Stems of Pueraria lobata and Their Tyrosinase Inhibitory Activity

• Morgan AM
• Jeon MN
• Jeong MH
• Yang SY
• Kim YH

• KMID: 2328876
• DOI: http://doi.org/10.20307/nps.2016.22.2.111

Abstract

Phytochemical investigation of the stems of Pueraria lobata (Wild) Ohwi (Leguminosae), led to the isolation of eighteen known compounds: β-amyrone (1), (+)-pinoresinol (2), (+)-syringaresinol (3) (+)-syringaresinol-O-β-D-glucoside (4), (+)-lariciresinol (5), (-)-tuberosin (6), naringenin (7), liquiritigenin (8), isoliquiritigenin (9) genistein (10), daidzein (11) daidzin (12) daidzein 4',7-diglucoside (13) 2,4,4'-trihydroxy deoxybenzoin (14), S-(+)-1-hydroxy-3-(4-hydroxyphenyl)-1-(4-hydroxy-2-methoxy-phenyl)propan-2-one (15), methyl 2-O-β-D-glucopyranosylbenzoate (16), pyromeconic acid 3-O-β-D-glucopyranoside 6'-(O-4''-hydroxy-3-methoxybenzoate) (17), and allantion (18). The chemical structures of these compounds were elucidated from spectroscopic data and by comparison of those data with previously published results. The effects of isolated compounds on mushroom tyrosinase enzymatic activity were screened. The results indicated that, chloroform extract of P. lobata stems turned out to be having tyrosinase inhibitory effect, and only compounds 5, 8, 9, and 11 showed enzyme inhibitory activity, with ICâ‚…â‚€ values of 21.49 ± 4.44, 25.24 ± 6.79, 4.85 ± 2.29, and 17.50 ± 1.29 µM, respectively, in comparison with these of positive control, kojic acid (ICâ‚…â‚€ 12.28 ± 2.72 µM). The results suggest that P. lobata stems extract as well as its chemical components may represent as potential candidates for tyrosinase inhibitors.

Keyword

Pueraria lobata; Leguminosae; Tyrosinase; Enzymatic activity; Inhibition

MeSH Terms

Agaricales
Chloroform
Fabaceae
Genistein
Monophenol Monooxygenase*
Pueraria*
Chloroform
Genistein
Monophenol Monooxygenase

Figure

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  Fig. 1. Chemical structures of isolated compounds (1–18).

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  Fig. 2. Tyrosinase inhibitory activity of extracts obtained from stems of P. lobata.

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  Fig. 3. Tyrosinase inhibitory activity of isolated compounds (1–18).

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