J Bacteriol Virol.  2016 Jun;46(2):57-62. 10.4167/jbv.2016.46.2.57.

Inhibitory Effects of D-mannose on Streptococcus mutans in the Presence of Sucrose

Affiliations
  • 1Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul, Korea. geji@snu.ac.kr
  • 2Research Institute, BIFIDO Co., Ltd., Hongchun, Gangwon, Korea.

Abstract

This study aimed to examine the inhibitory effect of rare sugars on Streptococcus mutans (S. mutans) in the presence of sucrose. Xylitol and three rare sugars (D-xylose, D-lyxose and D-mannose) were used in this study. S. mutans KCTC 3065 was cultured in Brain Heart Infusion (BHI) medium containing xylitol, D-xylose, D-lyxose, or D-mannose in the presence of sucrose, and the effect on S. mutans growth was assessed by measuring solution turbidity at different time points after inoculation. To assess effects on pH, sucrose was added at different concentrations, and solution pH was measured at different time points after inoculation. All sugars significantly inhibited the growth of S. mutans in the presence of sucrose. Especially, D-lyxose and D-mannose exhibited significantly greater inhibition than that of xylitol. Furthermore, unlike D-lyxose, D-mannose significantly inhibited the decrement of pH, and its effect was greater than that of xylitol. Taken together, D-mannose has strong inhibitory effect on S. mutans in the presence of sucrose.

Keyword

Streptococcus mutans; D-mannose; D-lyxose; Xylitol; Dental caries

MeSH Terms

Brain
Carbohydrates
Dental Caries
Heart
Hydrogen-Ion Concentration
Mannose*
Streptococcus mutans*
Streptococcus*
Sucrose*
Xylitol
Xylose
Carbohydrates
Mannose
Sucrose
Xylitol
Xylose

Figure

  • Figure 1. The inhibition of S. mutans growth by rare sugars in the presence of sucrose. (A) The turbidity of the solutions at 6, 12,18, 24, and 36 h after inoculation. (B) The ability of rare sugars to inhibit S. mutans growth at 12 h after inoculation. ∗p < 0.05 for the mean values vs. the control group (only contained sucrose). ∗∗p <0.05 for the mean values vs. the xylitol group.

  • Figure 2. The effect of various rare sugars on pH change of the growth medium containing sucrose at 12 h after inoculation. ∗ p < 0.05 for the mean values vs. the control group (only contained sucrose). ∗∗ p < 0.05 for the mean values vs. the xylitol group.


Reference

1). Guggenheim B. Extracellular polysaccharides and microbial plaque. Int Dent J. 1970; 20:657–78.
2). Ooshima T, Osaka Y, Sasaki H, Osawa K, Yasuda H, Matsumura M, et al. Caries inhibitory activity of cacao bean husk extract in in-vitro and animal experiments. Arch Oral Biol. 2000; 45:639–45.
3). Forbord B, Osmundsen H. On the mechanism of xylitol-dependent inhibition of glycolysis in Streptococcus sobrinus OMZ 176. Int J Biochem. 1992; 24:509–14.
4). Lindley MG, Birch GG, Khan R. Sweetness of sucrose and xylitol. Structural considerations. J Sci Food Agric. 1976; 27:140–4.
Article
5). Tapiainen T, Kontiokari T, Sammalkivi L, Ikaheimo I, Koskela M, Uhari M. Effect of xylitol on growth of Streptococcus pneumoniae in the presence of fructose and sorbitol. Antimicrob Agents Chemother. 2001; 45:166–9.
6). Kakuta H, Iwami Y, Mayanagi H, Takahashi N. Xylitol inhibition of acid production and growth of mutans Streptococci in the presence of various dietary sugars under strictly anaerobic conditions. Caries Res. 2003; 37:404–9.
Article
7). Winkelhausen E, Kuzmanova S. Microbial conversion of D-xylose to xylitol. J Ferment Bioeng. 1998; 86:1–14.
Article
8). Gong CS, Chen LF, Tsao GT. Quantitative production of xylitol from D-xylose by a high-xylitol producing yeast mutant Candida tropicalis HXP2. Biotechnol Lett. 1981; 3:125–30.
9). Takagi Y, Nakai K, Tsuchiya T, Takeuchi T. A 5′-(trifluoromethyl)anthracycline glycoside: synthesis of antitumor-active 7-O-(2, 6-dideoxy-6, 6, 6-trifluoro-alpha-L-lyxo-hexopyranosyl) adriamycinone. J Med Chem. 1996; 39:1582–8.
10). Patel DH, Wi SG, Lee SG, Lee DS, Song YH, Bae HJ. Substrate specificity of the Bacillus licheniformis lyxose isomerase YdaE and its application in in vitro catalysis for bioproduction of lyxose and glucose by two-step isomerization. Appl Environ Microbiol. 2011; 77:3343–50.
11). Davis JA, Freeze HH. Studies of mannose metabolism and effects of long-term mannose ingestion in the mouse. Biochim Biophys Acta. 2001; 1528:116–26.
Article
12). Martin-Villa MC, Vidal-Valverde C, Rojas-Hidalgo E. Soluble sugars in soft drinks. Am J Clin Nutr. 1981; 34:2151–3.
Article
13). Nystrom T. Stationary-phase physiology. Annu Rev Microbiol. 2004; 58:161–81.
14). Mickenautsch S, Yengopal V. Effect of xylitol versus sorbitol: a quantitative systematic review of clinical trials. Int Dent J. 2012; 62:175–88.
Article
15). Mickenautsch S, Yengopal V. Anticariogenic effect of xylitol versus fluoride – a quantitative systematic review of clinical trials. Int Dent J. 2012; 62:6–20.
Article
16). Honkala S, Runnel R, Saag M, Olak J, Nommela R, Russak S, et al. Effect of erythritol and xylitol on dental caries prevention in children. Caries Res. 2014; 48:482–90.
Article
17). Bader JD, Vollmer WM, Shugars DA, Gilbert GH, Amaechi BT, Brown JP, et al. Results from the Xylitol for Adult Caries Trial (X-ACT). J Am Dent Assoc. 2013; 144:21–30.
Article
18). Shallenberger RS. Hydrogen Bonding and the Varying Sweetness of the Sugars. J Food Sci. 1963; 28:584–9.
Article
19). Shallenberger RS, Acree TE, Guild WE. Configuration, conformation, and sweetness of hexose anomers. J Food Sci. 1965; 30:560–3.
Article
20). Stewart RA, Carrico CK, Webster RL, Steinhardt RG Jr. Physicochemical stereospecificity in taste perception of -D-mannose and -D-mannose. Nature. 1971; 234:220.
Article
21). Reid SJ, Abratt VR. Sucrose utilisation in bacteria: genetic organisation and regulation. Appl Microbiol Biotechnol. 2005; 67:312–21.
Article
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