J Nutr Health.  2015 Apr;48(2):149-156. 10.4163/jnh.2015.48.2.149.

Effects of combined intervention of isoflavone supplementation and exercise on bone metabolism in growing rats

Affiliations
  • 1Department of Food and Nutrition, Keimyung University, Daegu 704-701, Korea. choimj@kmu.ac.kr

Abstract

PURPOSE
This study examined the effects of combined intervention of isoflavones and exercise on bone mineral density, bone mineral content, and biochemical bone markers.
METHODS
Forty rats were divided into four groups; Control, Isoflavones (IF), Isoflavones + Running (IFR), and Isoflavones + Swimming (IFS). All of the rats in this study were fed an experimental diet and deionized water ad libitum for nine weeks. Bone mineral density (BMD) and bone mineral content (BMC) were estimated using PIXImus (GE Lunar Co, Wisconsin.) in spine and femur nine weeks after feeding or training.
RESULTS
The combined intervention did not affect weight gain, mean food intake, or food efficiency ratio. The serum concentrations of ALP and osteocalcin were not significantly different by combined intervention. The urinary DPD crosslinks values were not significantly different by combined intervention. There were no significant differences in serum PTH, calcitonin, and estradiol among all groups. Spine BMD, spine BMC and femur BMC were not significantly different by combined intervention. However, femur BMD was significantly higher in the IFR group than in the control group. Compared with the control group, spine BMD, spine BMC, and femur BMD per weight were markedly increased in the isoflavones supplementation and combined intervention group. In addition, femur BMC per weight was significantly higher in the IFS group than in the control group. Compared with the isoflavones supplemented group, BMD and BMC were not significantly different by combined intervention.
CONCLUSION
It can be concluded that isoflavones supplementation or combined intervention of isoflavone and exercise had a beneficial effect on spine and femur peak bone mass in growing female rats.

Keyword

isoflavones; exercise; combined intervention; bone mineral density; bone mineral content

MeSH Terms

Animals
Bone Density
Calcitonin
Diet
Eating
Estradiol
Female
Femur
Humans
Isoflavones
Metabolism*
Osteocalcin
Rats*
Running
Spine
Swimming
Water
Weight Gain
Calcitonin
Estradiol
Isoflavones
Osteocalcin
Water

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