J Korean Soc Pediatr Endocrinol.  2002 Dec;7(2):184-189.

Effect of Growth Hormone on Death of Sertoli Cell

• Chung MJ
• Yu JH

Abstract

PURPOSE: Growth hormone(GH) receptors have been detected in Sertoli cells and clinical observations suggested the involvement of GH action(either indirectly via IGF-I or directly) on testicular function. This study is aimed at assessing the effect of GH on cell death during Sertoli cell culture.
METHODS
Sertoli cells(TM4, mouse, male, ATCC, USA) were cultured by using DMEM(Dulbecco' Modified Eagle' Medium, Biowhitteker, USA) and Fetal Bovine Serum(10%, GIBCO BRL, USA) with or without human recombinant growth hormone(Eutropin, LGCI, Korea) of different concentrations(10, 50, 100, 500 microgram/mL of media), in 34degress C, 5% CO2 incubator. After cultivation during 5 days, Sertoli cells were examined under light microscope(x400).
RESULTS
Number of survival Sertoli cell was remarkably increased in the GH treated(50 microgram/mL in media) group(62.6+/-12.4) compare to control(22.4+/-4.8)(P<0.05). Degree of survival Sertoli cell was increased proportionaly until 50 microgram/mL in concentration of GH in media, but decreased in higher than it.
CONCLUSION
GH has the protective effect on death of Sertoli cell and it depends on GH concentration.

Keyword

Sertoli cell death; Growth hormone

MeSH Terms

Animals
Cell Culture Techniques
Cell Death
Growth Hormone*
Humans
Incubators
Insulin-Like Growth Factor I
Male
Mice
Sertoli Cells
Growth Hormone
Insulin-Like Growth Factor I