J Korean Acad Oral Health.  2014 Sep;38(3):148-153. 10.11149/jkaoh.2014.38.3.148.

Evaluation of the safety of non-thermal atmospheric-pressure plasma in hairless mouse tissues

  • 1Department of Dental Hygiene, Kyungnam College of Information & Technology, Busan, Korea. kjy1@kit.ac.kr
  • 2Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan, Korea.
  • 3Department of Dental Hygiene, Dongseo University, Busan, Korea.


The aim of the present study was to evaluate the stability of non-thermal atmospheric-pressure plasma on Candida albicans in hairless mouse-2 (HRM-2) tissues.
HRM-2 mice were subjected to non-thermal atmospheric-pressure plasma jet treatment using an optical fiber probe and monitored using a thermometer. The skin of HRM-2 mice was treated with plasma jet for 0, 60, 180, and 300 s per day for 5 days. After plasma treatment, morphological changes in Candida albicans on the skin of these mice were examined using a scanning electron microscope. Biopsy of the plasma-treated skin was performed and the tissues were histologically analyzed using hematoxylin and eosin (H&E) and Masson's trichrome stains.
The scanning electron microscopic images revealed the morphological changes in the membrane structure of the plasma-treated Candida albicans. Histological analysis showed that non-thermal plasma treatment did not cause epidermal damage or tissue inflammation and did not significantly modify the collagen layers of the mouse skin.
The results of this study suggest that non-thermal atmospheric-pressure plasma might be safe and effective for clinical applications in the field of dentistry.


Candida albicans; Hematoxylin and eosin stain; Masson's trichrome stain; Non-thermal atmospheric pressure plasma; Scanning electron microscopy
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