Nutr Res Pract.  2012 Oct;6(5):389-395.

Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts of Rumex crispus

Affiliations
  • 1Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, 192-1 Hyoja-dong, Chuncheon-si, Gangwon 200-701, Korea. mhwang@kangwon.ac.kr
  • 2Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University, Gangwon 200-701, Korea.

Abstract

The study elucidated carbohydrase inhibition, anti-cancerous, free radical scavenging properties and also investigated the DNA and protein protection abilities of methanolic root extract of Rumex crispus (RERC). For this purpose
, pulverized roots of Rumex crispus was extracted in methanol (80% and absolute conc.) for 3 hrs for 60degrees C and filtered and evaporated with vacuum rotary evaporator. RERC showed high phenolic content (211 microg/GAE equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (IC50 = 42.86 (absolute methanol) and 36.91 microg/mL (80% methanolic extract)) and reduced power ability. Furthermore, RERC exhibited significant protective ability in H2O2/Fe3+/ascorbic acid-induced protein or DNA damage and percentage inhibition of the HT-29 cell growth rate following 80% methanolic RERC exposure at 400 microg/mL was observed to be highest (10.2% +/- 1.03). Moreover, methanolic RERC inhibited alpha-glucosidase and amylase effectively and significantly (P < 0.05). Conclusively, RERC could be considered as potent carbohydrase inhibitor, anti-cancerous and anti-oxidant.

Keyword

Root of Rumex crispus; methanolic extract; antioxidative; carbohydrase inhibitor; anti-cancerous
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