Nat Prod Sci.  2015 Dec;21(4):261-267. 10.20307/nps.2015.21.4.261.

Deuteromethylactin B from a Freshwater-derived Streptomyces sp.

Affiliations
  • 1Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Chicago, IL 60612, USA. btmurphy@uic.edu
  • 2Center for Biomolecular Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
  • 3Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
  • 4DNA Services Facility, University of Illinois at Chicago, Chicago, IL, 60612, USA.

Abstract

Compared to their terrestrial and marine counterparts, little is known about the capacity of freshwater-derived actinomycete bacteria to produce novel secondary metabolites. In the current study, we highlight the disparities that exist between cultivation-independent and -dependent analyses of actinomycete communities from four locations in Lake Michigan sediment. Furthermore, through phylogenetic analysis of strains isolated from these locations, we identified a Streptomyces sp., strain B025, as being distinct from other Streptomyces spp. isolated from sediment. Upon fermentation this strain produced a rare class of eight-membered lactone secondary metabolites, which have been for their antitumor properties. We used spectroscopic and chemical derivitization techniques to characterize octalactin B (1) in addition to its corresponding novel, unnatural degradation product (2).

Keyword

Actinomycete; Freshwater; Streptomyces; Octalactin

MeSH Terms

Bacteria
Fermentation
Fresh Water
Lakes
Michigan
Streptomyces*
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