Korean J Neurogastroenterol Motil.  2005 Dec;11(2):152-160.

Changes of Colonic Neuromuscular Function and Mophometry in Aged Rats

Affiliations
  • 1Department of Internal Medicine, Ewha Womans University College of Medicine, Ewha Medical Research Institute, Seoul, Korea. jassa@mm.ewha.ac.kr
  • 2Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND/AIMS
The aim of this study was to investigate the alteration of neuromuscular function and the morphometric changes of the nervous and fibrous tissue in the aged rat colon.
METHODS
We used old Sprague-Dawley female rats (n=33, over 20 months), and young rats (n=28, 8-10 weeks). Glass bead expulsion tests were done in vivo, and the colon transit time was measured using a peristaltic bath in vitro. Immunohistochemical staining of PGP-9.5 for the nervous tissue and c-kit for the ICC were performed in the colonic muscle. Collagen quantification was done in each group by employing a spectrophotometer.
RESULTS
For the glass bead expulsion, significantly more time was needed for their transit in the young group (p=0.028). The number of rats with complete transit was greater in the young group. The density and area of the nervous tissue and the ICC, as determined by immunostaining were not different between the two groups. On the collagen analysis, a significantly higher level of collagen was detected in the old rats (p=0.02).
CONCLUSIONS
The old rats showed a higher incidence of incomplete transit and an increase in their fibrotic tissue. These findings might reflect the degenerative aging process in the colon.

Keyword

Old age; Colon transit; Nervous tissue

MeSH Terms

Aging
Animals
Baths
Collagen
Colon*
Female
Glass
Humans
Incidence
Rats*
Rats, Sprague-Dawley
Collagen
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