Hemodynamic changes and histopathologic findings of the kidney following multiple renal punctures or renal viopsies in rats

Kim, Tae Jin; Park, Tong Choon

Abstract

To investigate the hemodynamic changes and histopathologic findings of the kidney following multiple renal punctures or renal biopsies in rats the measurement of arterial pressure and plasma renin level, renal angiography and histopathologic studies were performed. The 40 male Sprague-Dawley rats divided into 4 groups; control group (n=10, sham operation), group 1 (n=10, five consecutive renal punctures1. group 2 (n=10, ten consecutive renal punctures) and group 3 (n=10. two consecutive renal biopsies). The results obtained were summarized as follows; the arterial pressure and plasma renin levels after operations were significantly different from those before operations in the group 1, 2 and 3 (p<0.01) but in the control group there was no significant difference. The changes of arterial pressure in the group 3 were markedly fluctuated in each case after operation. The renal angiography was performed in 28 rats; in the group 1 (n=9) avascular areas were demonstrated in 6 cases, in the group 2 (n=7) avascular areas were demonstrated in 5 cases and intrarenal arteriovenous fistulae in 2 cases, in the group 3 (n=7) avascular areas were demonstrated in 3 cases and intrarenal arteriovenous fistulae in 1 case. On the histopathologic studies, atrophy of the renal cortex and infiltration of inflammatory cells around the renal injury site were observed in most cases and these findings were more prominent in the group 2. More severe fibrosis was found in the group 3 than the group 1 or 2. The renal glomerular and tubular injuries were more prominent in the group 2 than the group 1 or 3. The intrarenal arteriovenous fistulae were demonstrated in only three cases of the group 2 under Verhoeff-elastic fiber stain. Therefore, it is postulated that the more frequent renal puncture would induce the more significant hemodynamic changes and the more profound histopathologic findings.