J Asthma Allergy Clin Immunol.
1999 Oct;19(5):666-676.
Effects of Cortex morl on the activation of rat peritoneal mast cells
by human seminal plasma
Abstract
- BACKGROUND
It has been reported that Cortex mori ( Morus alba L,
Sangbaikpi ; CM ), the root bark of mulberry tree, plays a role in inhibiting mast
cell activation. Human seminal plasma ( HSP ) modulates immune systems and activates
rat peritoneal mast cells ( RPMC ). However, the inhibitory agents of HSP - induced
mast cell activation have not been disclosed yet.
OBJECTIVE
AND METHOD: This study was undertaken to determine the effects of CM on
HSP-induced mast cell activation by morphologic and functional methods. Morphological
changes of RPMC by HSP alone or CM plus HSP were observed with the inverted light
microscope. And the amounts of histamine and calcium were measured by radioisotopic
enzymatic histamine and radioisotopic calcium assays.
RESULTS
By inverted microscopy, HSP-induced RPMC degranulation occurred
in a dose-dependent fashion. After pretreatment of RPMC with CM, there was no
HSP-induced degranulation. Degranulation index (DI) of RPMC treated with Hanks
balanced salt solution (HBSS), CM or HSP was 7.0+/-4.3, 9.0+/-6.2, 56.5+/-16.9, respectively.
After pretreatment of RPMC with CM, HSP-induced DI was 11.0 +/-7.1.
These results indicate that CM inhibited HSP-induced degranulation of RPMC.
HSP-induced RPMC histamine release (HR) was dose-dependent. HR of RPMC treated with HBSS,
CM or HSP was 0.18+/-0.10, 0.30+/-0.19, 4.48+/-0.86 ug/ml, respectively. After pretreatment
of RPMC with CM, HSP-induced HR was 0.52+/-0.21 ug/ml. These results indicate that
CM inhibited HSP-induced HR from RPMC. Intracellular calcium level ( ICL) of RPMC
was also increased according to the concentration of HSP. ICL of RPMC treated with HBSS,
CM or HSP was 6.1+/-1.0, 9.0+/-2.1, 30.2+/-6.5 pmole, respectively. After pretreatment of
RPMC with CM, HSP-induced ICL of RPMC was 11.6 +/- 3.3 pmole. These results indicate that
CM inhibited HSP-induced calcium uptake of RPMC.
CONCLUSION
From these results, it is suggested that CM contains some substances
which inhi-