Korean J Pediatr Hematol Oncol.  2003 Oct;10(2):262-268.

The Effect of Vasoactive Intestinal Peptide on Cord Blood CD34 (+) Cells

• Yeo MK
• Bae HY
• Hur MK
• Ra JS
• Han IS
• Kim MK
• Kim SK
• Park SK

Abstract

PURPOSE
We investigated the expression of vasoactive intestinal peptide (VIP), VIP receptor 1 (VPAC1), VIP receptor 2 (VPAC2) genes in the human umbilical cord blood CD34 cells, and the ability of VIP to stimulate human primitive as well as monopotent hematopoietic progenitors. METHODS: We isolated RNA from umbilical cord blood CD34 cells, and then performed RT-PCR, and sequencing. The umbilical cord blood CD34 cells were cultured with the various concentrations of VIP for burst-forming unit of erythrocyte (BFU-E), colony-forming unit of granulocyte/monocyte (CFU-GM), colony-forming unit of graulocyte/erythrocyte/monocyte/megakaryocyte (CFU-GEMM), and colony-forming unit of megakaryocyte (CFU-Mk). RESULTS: The RNA coding for VPAC1 was detected in human umbilical cord blood CD34 cells. VIP significantly stimulated the growth of CFU-GEMM and CFU-Mk. CONCLUSION: The present results suggest that VIP is an important neuropeptide in the early proliferation of human primitive as well as megakaryocyte progenitors.

Keyword

Vasoactive intestinal peptide; Vasoactive intestinal peptide receptor; Umbilical cord blood; CD34 cells; CFU-GEMM; CFU-Mk

MeSH Terms

Clinical Coding
Erythrocytes
Fetal Blood*
Humans
Megakaryocyte Progenitor Cells
Megakaryocytes
Myeloid Progenitor Cells
Neuropeptides
Receptors, Vasoactive Intestinal Peptide
RNA
Stem Cells
Vasoactive Intestinal Peptide*
Neuropeptides
RNA
Receptors, Vasoactive Intestinal Peptide
Vasoactive Intestinal Peptide