Chonnam Med J.  1997 Dec;33(2):213-220.

Contractile Effects of Cholecystokinin on the Muscle Strips of Gallstone Disease

• Rew JS
• Choi SK
• Kim SJ
• Yoon CM
• Kim HJ

Abstract

Cholecystokinin(CCK), a hormone and potential neurotransmitter that is released after meals, plays an important role in the control of gallbladder(GB) contractility. Decreased contractility of the GB is believed as a major pathogenetic factor in gall stone disease. We evaluated the effect of CCK on the contractile function of the GB muscles strips of gall stone disease. Isometric tension studied were performed in the surgically resected GB of the gallstone disease. Muscle strips of 3x8mm along the long axis of the GB were prepared on the petridish containing Krebs bicarbonate buffer gassed with 5% CO2 and 95% O2. Muscle strips were suspended in 10cc organ baths and recordings were graphed on a rectilinear polygraphy(Grass Instruments Co.). Contractile response of the muscle strips was expressed as a percentage of control maximum cholinergic(bethanechol 10-5mol/L) response. CCK-8 increased the contractile activity of GB muscle strips dose dependently. Contractile response of CCK-8 was not suppressed by the atropine(10-5mol/L) and propranolol(10-6mol/L) pretreatment. L-arginine(10-2mol/L) inhibited the CCK-8(10-8mol/L) induced contraction by 49% and it was reversed after pretreatment of LNNA, nitric oxide synthase inhibitor. Vasoactive intestinal peptide(VIP, 10-7mol/L) also inhibited the CCK-8(10-8mol/L) induced contraction by 38%. In conclusions, CCK-induced contraction of human GB was affected in part by L-arginine nitric oxide pathway and VIP.

Keyword

Cholecystokinin; Isometric contraction; Galbladder; CCK

MeSH Terms

Arginine
Axis, Cervical Vertebra
Baths
Cholecystokinin*
Gallstones*
Humans
Isometric Contraction
Meals
Muscles
Neurotransmitter Agents
Nitric Oxide
Nitric Oxide Synthase
Sincalide
Arginine
Cholecystokinin
Neurotransmitter Agents
Nitric Oxide
Nitric Oxide Synthase
Sincalide