Korean J Obstet Gynecol.  2007 Jan;50(1):101-110.

Establishment and characterization of secondary drug resistant cell line originating from cisplatin-resistant SNU-8

• An CS
• Kim SY

Abstract

OBJECTIVE
Multidrug resistance to chemotherapy is a major obstacle in attempts to improve the clinical outcome of ovarian carcinoma patients. The aim of this study is to establish secondary anticancer drug resistant cell line from original SNU-8/WT resistant to cisplatin and characterize it.
METHODS
After establishing secondary drug resistant cell line (SNU-8/Fac), drug sensitivity was measured by MTT assay. Multidrug resistance (MDR) was analyzed by RT-PCR and western blotting analysis.
RESULTS
MTT assay data demonstrated that MDR was expressed in SNU-8/Fac. In addition, mRNA expression of MDR1, ATP7B in SNU-8/Fac was increased. However, overexpression of MRP1, BCRP, TS and MT mRNA was not observed. At the protein level, protein P-gp, ATP7B were overexpressed in SNU-8/Fac.
CONCLUSION
We established a new anticancer drug resistant cell line from original SNU-8/WT. Overexpression of P-gp and ATP7B was observed in SNU-8/Fac.

Keyword

Ovarian cancer; Multidrug resistance; P-gp/MDR1; ATP7B; Cisplatin

MeSH Terms

Blotting, Western
Cell Line*
Cisplatin
Drug Resistance, Multiple
Drug Therapy
Humans
Ovarian Neoplasms
P-Glycoprotein
RNA, Messenger
Cisplatin
P-Glycoprotein
RNA, Messenger