Ann Dermatol.  2009 Aug;21(3):237-242. 10.5021/ad.2009.21.3.237.

A Global Gene Expression Analysis of the Peripheral Blood Mononuclear Cells Reveals the Gene Expression Signature in Psoriasis

• Lee SK
• Jeon EK
• Kim YJ
• Seo SH
• Kim CD
• Lim JS
• Lee JH

• KMID: 2266283
• DOI: http://doi.org/10.5021/ad.2009.21.3.237

Abstract

BACKGROUND: Psoriasis is a chronic inflammatory skin disease that affects approximately 1~3% of the general population.
OBJECTIVE
We performed cDNA microarray analysis with using the dendrimer labelling method to investigate the gene expression profile in the peripheral blood mononuclear cells (PBMCs) of psoriatic patients.
METHODS
The peripheral blood mononuclear cells of 5 patients with psoriasis and 8 control subjects were used in the gene expression analyses of psoriasis.
RESULTS
We identified 212 differentially expressed genes that showed at least a two-fold induction and/or reduction in psoriatic patients. Among those, 63 genes, including CD44, CD56 and IL7R, were induced, while 139 genes, including the sphingosine kinase 1 and p16-INK genes, were reduced in the psoriatic patients.
CONCLUSION
We can speculate that these genes may have a role for the pathogenesis of psoriasis via their affecting different cellular functions. Our results suggest a possible mechanism by which activated immune cells migrate from the blood to the skin in psoriatic patients, and we provide novel putative targets for developing drugs to treat psoriasis

Keyword

Cell adhesion; Microarray; Peripheral blood mononuclear cells; Psoriasis

MeSH Terms

Cell Adhesion
Dendrimers
Gene Expression
Humans
Oligonucleotide Array Sequence Analysis
Phosphotransferases
Phosphotransferases (Alcohol Group Acceptor)
Psoriasis
Skin
Skin Diseases
Sphingosine
Transcriptome
Dendrimers
Phosphotransferases
Phosphotransferases (Alcohol Group Acceptor)
Sphingosine

Figure

• 

  Fig. 1 The gene expression profile of the PBMCs from the 5 psoriatic patients. Each row represents a gene; each column shows the expression for the 212 genes expressed by each individual. The color red indicates the up-regulated genes in the psoriatic patients, while the color green indicates the down-regulated genes in the psoriatic patients.

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