Korean J Blood Transfus.  2000 Jun;11(1):49-55.

Microbial Contamination of Autologous Peripheral Blood Stem Cell

Affiliations
  • 1Department of Clinical Pathology, Kosin University College of Medicine, Pusan, Korea.
  • 2Department of Internal Medicine, Kosin University College of Medicine, Pusan, Korea.
  • 3Department of Pediatrics, Kosin University College of Medicine, Pusan, Korea.

Abstract

BACKGROUND
The incidence and clinical consequences of microbiological contamination of autologous peripheral blood stem cells are not well documented. Therefore, we retrospectively analysed our experience with microbial contamination of autologous peripheral blood stem cell concentrates. METHOD: We have determined the incidence and clinical significance of positive microbiologic cultures in series of 52 peripheral blood stem cell concentrates in 14 patients undergoing multiple apheresis procedures for autologous stem cell rescue. Specimens for bacterial cultures were obtained after processing of the autografts just prior to freezing. RESLUTS: The incidence of microbial contamination was 7.7%. The microorganism cultured was coagulase negative Staphylococcus. The patient with contaminated leukapheresis products received two culture-positive stem cell concentrates and three culture-negative stem cell concentrates without adverse clinical sequelae. No microorganism present in the stem cell autograft was recovered in vivo in the posttransplantation period, although fever as a sign of infection occurred in this patient.
CONCLUSIONS
Although microbial contamination may occur during autologous peripheral blood stem cell collection and cryopreservation, this report suggests that peripheral blood stem cell contamination may not play a significant role in the infectious complications of autologous peripheral blood stem cell transplantation.

Keyword

Microbial contamination; Autologous peripheral blood stem cell; transplantation
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