Korean J Lab Med.  2006 Apr;26(2):114-118. 10.3343/kjlm.2006.26.2.114.

Gene Frequencies of Human Neutrophil Antigens 4a and 5a in the Korean Population

• Han TH
• Han KS

• KMID: 2238901
• DOI: http://doi.org/10.3343/kjlm.2006.26.2.114

Abstract

BACKGROUND: In Korean population, antigen frequencies of HNA-1a, HNA-1b, and HNA-2a are determined using a serological and genotyping method. However, no study has been done to assess the gene frequencies of HNA-4a and HNA-5a. It has been reported that the antibody against HNA-4a is associated with alloimmune neutropenia and autoimune neutropenia; however, there is no confirmed clinical report on anti-HNA-5a-related disorders. The aim of this study was to determine HNA-4a and HNA-5a gene frequencies among the Korean population.
METHODS
Genotyping of HNA-4a and HNA-5a genes of 110 healthy and unrelated Korean donors was performed using a polymerase chain reaction with sequence-specific primers and an allelespecific restriction enzyme analysis.
RESULTS
We found that the gene frequencies of HNA-4a and HNA-5a were 0.99 (107/110) and 0.96 (3/110), respectively, among the Korean population. But only the ones of the latter was significantly higher (P<0.01) than in the one of Caucasians.
CONCLUSIONS
The gene frequencies of HNA-4a and HNA-5a were determined. We also identified an individual who was the HNA-5a-negative homozygote for the granulocyte panel that could be used for anti-HNA-5a antibody identification.

Keyword

HNA-4a antigen; HNA-5a antigen; Gene frequency

MeSH Terms

Gene Frequency*
Granulocytes
Homozygote
Humans*
Neutropenia
Neutrophils*
Polymerase Chain Reaction
Restriction Mapping
Tissue Donors

Figure

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  Fig. 1. HNA-4a genotyping by PCR-SSP. Lanes 1 and 6 show a DNA ladder marker (Bioneer, Daejeon, Korea). The amplification products (439 bp) of the internal control (HGH gene) are present in each lane. Lanes 2, 3, 7, and 8 contain HNA-4a-positive-specific reactions. Lanes 4, 5, 9, and 10 contain HNA-4a-negative-specific reactions. Lanes 2 and 4 are positive controls for HNA-4a-positive and HNA-4a-negative samples, respectively, and lanes 3 and 5 are negative controls for HNA-4a-positive and HNA-4a-negative samples, respectively. Lanes 7 and 8 contain an HNA-4a-positive homozygote sample and lanes 9 and 10 contain an HNA-4a-heterozygote sample.

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  Fig. 2. HNA-5a genotyping by Bsp1286I allele-specific restriction enzyme analysis (ASRA). Lane 1 shows a DNA ladder marker (Bioneer, Daejeon, Korea); lane 2 shows an undigested 709 bp PCR product of αL chain of β2 integrin cDNA; lane 3 shows an HNA-5a-positive homozygote sample (297 bp, 217 bp, and 195 bp); lane 4 shows an HNA-5a heterozygote sample (412 bp, 297 bp, 217 bp, and 195 bp); and lane 5 shows an HNA-5a-negative homozygote sample (412 bp, and 297 bp).

Reference

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