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A Case of Cutaneous Leukocytoclastic Vasculitis Associated with Granulocyte Colony-Stimulating Factor: An Unusual Presentation

Yoon D, Byun HJ, Oh SJ, Park JH, Lee DY

Drug-induced vasculitis is an inflammation of small-sized blood vessel caused by the use of drugs. It accounts for approximately 10% of acute cutaneous vasculitis. Propylthiouracil, hydralazine, and allopurinol have been...
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Delta Neutrophil Index as an Early Marker of Sepsis in Burn Patients

Kim CM, Ha CM

PURPOSE: The immature granulocyte count has been reported to be a marker of infection and sepsis. The difference in leukocyte subfractions (delta neutrophil index, DNI) in ADVIA 2120 reflects the...
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Clonal Cell Proliferation in Paroxysmal Nocturnal Hemoglobinuria: Evaluation of PIGA Mutations and T-cell Receptor Clonality

Park J, Kim M, Kim Y, Han K, Chung NG, Cho B, Lee SE, Lee JW

BACKGROUND: Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired pluripotent hematopoietic stem cell disorder associated with an increase in the number of glycosyl-phosphatidyl inositol (GPI)-deficient blood cells. We investigated PNH clonal...
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Comparison of the virulence of Streptococcus pneumoniae in ICR mouse stocks of three different origins

Kim JY, Seo SM, Lee HK, Kim HW, Choi YK

Streptococcus pneumoniae causes many people to suffer from pneumonia, septicemia, and other diseases worldwide. To identify the difference in susceptibility of and treatment efficacy against S. pneumoniae in three ICR...
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Evaluation of Time and Temperature Stability of Paroxysmal Nocturnal Hemoglobinuria Cells by Flow Cytometry

Lee SH, Bang HI, Shin YJ, Shin WY, Kim J, Park R, Shin JW, Choi TY

BACKGROUND: Flow cytometry analysis of paroxysmal nocturnal hemoglobinuria (PNH) is significantly affected by the methodology used. The lack of data on the effect of age and refrigeration on PNH clone...
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Analysis of hematologic parameters of donors, patients, and granulocyte concentrates to predict successful granulocyte transfusion

Lee JM, Choi SJ, Kim HS, Yang M, Kim Y, Lee JW, Lim J

BACKGROUND: Granulocyte transfusion (GTx) is performed as a supportive therapy in severe neutropenic patients caused by various conditions. The study aimed to analyze the hematologic parameters of donors, patients, and...
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Hematopoiesis Activity of Sambucus javanica on Chloramphenicol-induced Aplastic Anemia Mouse Model

Putra WE, Rifa'i M

Hematopoiesis has a pivotal role in the maintenance of body homeostasis. Ironically, several hematological disorder caused by chemicals, drugs, and other environmental factors lead to severe bone marrow failure. Current...
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Comparison of High Sensitivity and Conventional Flow Cytometry for Diagnosing Overt Paroxysmal Nocturnal Hemoglobinuria and Detecting Minor Paroxysmal Nocturnal Hemoglobinuria Clones

Park SH, Jeong J, Lee SH, Yoo DW, Choi Y, Jo JC, Lim JH

BACKGROUND: High sensitivity flow cytometry (HS-FCM) was recently developed for diagnosing paroxysmal nocturnal hemoglobinuria (PNH). We compared its performance with conventional flow cytometry (C-FCM) for diagnosing overt PNH and detecting...
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Development of a Novel Flow Cytometry-Based System for White Blood Cell Differential Counts: 10-color LeukoDiff

Park D, Chang J, Kahng J, Park H, Jo I, Kim Y, Han K

BACKGROUND: Flow cytometry (FCM) is commonly used to identify many cell populations. We developed a white blood cell (WBC) differential counting system for detecting abnormal cells using FCM incorporating 10...
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Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Korea

Seo MG, Ouh IO, Choi E, Kwon OD, Kwak D

The identification and characterization of pathogenic and zoonotic tick-borne diseases like granulocytic anaplasmosis are essential for developing effective control programs. The differential diagnosis of pathogenic Anaplasma phagocytophilum and non-pathogenic A....
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Antiviral and Anti-Inflammatory Activities of Pochonin D, a Heat Shock Protein 90 Inhibitor, against Rhinovirus Infection

Song JH, Shim A, Kim YJ, Ahn JH, Kwon BE, Pham TT, Lee J, Chang SY, Ko HJ

Human rhinoviruses (HRV) are one of the major causes of common cold in humans and are also associated with acute asthma and bronchial illness. Heat-shock protein 90 (Hsp90), a molecular...
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Dapsone modulates lipopolysaccharide-activated bone marrow cells by inducing cell death and down-regulating tumor necrosis factor-α production

Kwon MJ, Joo HG

Dapsone, an antibiotic, has been used to cure leprosy. It has been reported that dapsone has anti-inflammatory activity in hosts; however, the anti-inflammatory mechanism of dapsone has not been fully...
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Delta Neutrophil Index as an Early Marker for Distinguishing Myeloid from Childhood Acute Leukemia

Hong JP, Kim S, Kim BR, Yoon SH, Hahn SM, Kim MK

BACKGROUND: The accurate and early diagnosis of acute myeloid leukemia (AML) is important to choose proper treatment option depending on the risk stratification. The delta neutrophil index (DNI) is a...
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Serial Changes in Mannose-Binding Lectin in Patients with Sepsis

Huh JW, Song K, Kim HJ, Yum JS, Hong SB, Lim CM, Koh Y

BACKGROUND: Mannose-binding lectin (MBL) deficiency leads to increased susceptibility to infection. We investigated whether serial changes in MBL levels are associated with the prognosis of patients diagnosed with septic shock,...
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Flow-Cytometric Analysis of HNA-2 Expression and Phenotypes Among Thai Blood Donors

Nathalang , Siriphanthong K, Petvises S, Jeumjanya

BACKGROUND: Antibodies specific to human neutrophil antigen (HNA), especially HNA-2, are implicated in various conditions, including neonatal alloimmune neutropenia, febrile non-hemolytic transfusion reactions, and transfusion-related acute lung injury. The distribution...
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Busulfan plus melphalan versus high-dose melphalan as conditioning regimens in autologous stem cell transplantation for newly diagnosed multiple myeloma

Byun JM, Lee J, Shin SJ, Kang M, Yoon SS, Koh Y

BACKGROUND: High-dose melphalan (HDMEL) represents the standard conditioning regimen before autologous stem cell transplant (ASCT) in multiple myeloma (MM), but recent updates have suggested combination of melphalan with bulsulfan (BUMEL)...
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Investigation of Immune-Regulatory Effects of Mageumsan Hot Spring via Protein Microarray In Vitro

Hahn HJ, Kim JS, Kim YH, Lee YB, Yu DS, Kim JW

BACKGROUND: Empirical evidences for efficacy of hot spring (HS) water in inflammatory skin disorders have not been substantiated with sufficient, immunological “hard evidence”. Mageumsan HS water, characterized by its weakly-alkaline...
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Delta Neutrophil Index Is Associated with Vasculitis Activity and Risk of Relapse in ANCA-Associated Vasculitis

Yoo J, Ahn SS, Jung SM, Song JJ, Park YB, Lee SW

PURPOSE: Delta neutrophil index (DNI) represents the immature granulocytes count associated with neutrophil-consumption. We investigated whether DNI might be associated with Birmingham vasculitis activity score (BVAS) at diagnosis and could...
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Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells

Na HY, Sohn M, Ryu SH, Choi W, In H, Shin HS, Park CG

Bone marrow-derived dendritic cells (BM-DCs) are generated from bone marrow (BM) cells cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) for a week. In this study we investigated the effect of...
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A Prospective Study of Eosinophilic Esophagitis and the Expression of Tight Junction Proteins in Patients with Gastroesophageal Reflux Disease Symptoms

Okimoto K, Arai M, Ishigami H, Saito K, Minemura S, Maruoka D, Matsumura , Nakagawa T, Katsuno T, Suzuki M, Nakatani Y, Yokosuka O

BACKGROUND/AIMS: Eosinophilic esophagitis (EoE) is often erroneously diagnosed as gastroesophageal reflux disease (GERD). The aim of this study is to investigate the prevalence of EoE and the expression of tight...
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