J Adv Prosthodont.  2014 Apr;6(2):109-114. 10.4047/jap.2014.6.2.109.

Effect of denture cleansers on Candida albicans biofilm formation over resilient liners

Affiliations
  • 1Department of Prosthodontics, Busan National University, School of Dentistry, Dental Research Institute, Yangsan, Republic of Korea.
  • 2College of Arts and Science, New York University, NY, USA.
  • 3Department of Prosthodontics, Institute for Clinical Dental Research, Korea University Guro Hospital, Korea University, Seoul, Republic of Korea. swshin@korea.ac.kr
  • 4Maxillofacial Prosthodontics, Head and Neck Institute, Cleveland Clinic, Cleveland, OH, USA.

Abstract

PURPOSE
The purpose of this study was to analyze the effect of denture cleansers on Candida albicans biofilm formation over resilient liners and to evaluate compatibility between resilient liners and denture cleansers.
MATERIALS AND METHODS
Acrylic resin (Lucitone 199(R)) and 3 resilient liners (COE-SOFT(TM), GC RELINE(TM) and SOFRELINER TOUGH TOUGH(R)) were incubated in denture cleansers (Polident(R) and Cleadent(R)) for 8 hours a day and in unstimulated saliva for 16 hours a day (n=25/gp) for 60 days. Two-way and three-way repeated measures ANOVA were performed to compare the surface roughness (Ra), pH and C. albicans binding level by radioisotope (alpha=0.05). The statistical significance of the relation between Ra and adhesion was evaluated by correlation analysis.
RESULTS
The degree of Ra was significantly decreased in the following order: COE-SOFT(TM), acrylic resin, GC RELINE(TM) and SOFRELINER TOUGH(R). The immersion in denture cleansers significantly increased Ra of resilient liners, except for SOFRELINER TOUGH(R) in Cleadent(R). No significant differences in pH curves were observed among groups immersed in distilled water and denture cleansers. The binding levels of C. albicans were significantly decreased in the following order: COE-SOFT(TM), GC RELINE(TM), SOFRELINER TOUGH(R), and acrylic resin. The immersion in Cleadent(R) seemed to decrease C. albicans binding level on GC RELINE(TM) and SOFRELINER TOUGH(R).
CONCLUSION
Based on the C. albicans binding levels results, it is not recommended to immerse COE-SOFT(TM) in denture cleansers, and GC RELINE(TM) and SOFRELINER TOUGH(R) should be immersed in Cleadent(R).

Keyword

Candida albicans; Biofilm; Resilient liners; Denture cleanser

MeSH Terms

Biofilms*
Candida albicans*
Denture Cleansers*
Hydrogen-Ion Concentration
Immersion
Saliva
Water
Denture Cleansers
Water

Figure

  • Fig. 1 Average surface roughness (Ra) of acrylic resin and resilient liners immersed in denture cleansers (mm). Immersion in denture cleansers increased Ra of soft relining materials, except for SOFRELINER TOUGH® in Cleadent® (*P<.01).

  • Fig. 2 The pH curve of medium in which C. albicans grown on acrylic resin and resilient liners immersed in distilled water, Polident®, Cleadent®. No significant differences were observed among groups immersed in distilled water and denture cleansers (P>.05).

  • Fig. 3 Binding levels of radioisotope-labeled C. albicans on acrylic resin and resilient liners immersed in distilled water and denture cleanser. Except for GC RELINE™ and SOFRELINER TOUGH® immersed in Cleadent®, all the specimens immersed in denture cleansers exhibited significantly higher capacity of fungal biofilm formation than the control specimens (in distilled water) (*P<.01).


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