J Periodontal Implant Sci.  2012 Oct;42(5):166-172. 10.5051/jpis.2012.42.5.166.

Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with synthetic cell binding peptide sequences

• Choi H
• Park NJ
• Jamiyandorj O
• Hong MH
• Oh S
• Park YB
• Kim S

• KMID: 2212076
• DOI: http://doi.org/10.5051/jpis.2012.42.5.166

Abstract

PURPOSE
The aim of this study was to evaluate the improvement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with synthetic cell-binding peptide sequences in a standardized rabbit sinus model.
METHODS
Standardized 6-mm diameter defects were created bilaterally on the maxillary sinus of ten male New Zealand white rabbits, receiving BCP bone substitute coated with synthetic cell binding peptide sequences on one side (experimental group) and BCP bone substitute without coating (control group) on the other side. Histologic and histomorphometric analysis of bone formation was carried out after a healing period of 4 or 8 weeks.
RESULTS
Histological analysis revealed signs of new bone formation in both experimental groups (4- and 8-week healing groups) with a statistically significant increase in bone formation in the 4-week healing group compared to the control group. However, no statistically significant difference in bone formation was found between the 8-week healing group and the control group.
CONCLUSIONS
This study found that BCP bone substitute coated with synthetic cell-binding peptide sequences enhanced osteoinductive potential in a standardized rabbit sinus model and its effectiveness was greater in the 4-week healing group than in the 8-week healing group.

Keyword

Bone regeneration; Bone substitutes; Maxillary sinus; Oligopeptides; Rabbits

MeSH Terms

Artificial Cells
Bone Regeneration
Bone Substitutes
Calcium
Durapatite
Humans
Hydroxyapatites
Male
Maxillary Sinus
Oligopeptides
Osteogenesis
Rabbits
Bone Substitutes
Calcium
Durapatite
Hydroxyapatites
Oligopeptides

Figure

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  Figure 1 Histological findings of control group (biphasic calcium phosphate) at 4 weeks. (A) Area of interest (H&E, ×12.5), (B) defect margin area (H&E, ×50), (C) the Schneiderian membrane (H&E, ×50), and (D) middle area (H&E, ×50).

• 

  Figure 2 Histological findings of experimental group (biphasic calcium phosphate coated with oligopeptides) at 4 weeks. (A) Area of interest (H&E, ×12.5), (B) defect margin area (H&E, ×50), (C) the Schneiderian membrane (H&E, ×50), and (D) middle area (H&E, ×50).

• 

  Figure 3 Histological findings of the Schneiderian membrane in experimental group (biphasic calcium phosphate coated with oligopeptides) at 4 weeks. The Schneiderian membrane (H&E, ×50). More vascularization (white asterisks) with newly formed blood vessels (arrows) were identified in the 4-week healing group. Bone matrix, including osteoblasts (arrowheads), was also observed.

• 

  Figure 4 Histological findings of experimental group (biphasic calcium phosphate coated with oligopeptides) at 8 weeks. (A) Area of interest (H&E, ×12.5), (B) defect margin area (H&E, ×50), (C) the Schneiderian membrane (H&E, ×50), and (D) middle area (H&E, ×50). The Schneiderian membrane was thicker than that of the 4-week healing group. Also, the shape of graft materials was smoothened, which indicates the resorption of graft material particles.

• 

  Figure 5 Histological findings of middle area in experimental group (biphasic calcium phosphate coated with oligopeptides) at 8 weeks. Middle area (H&E, ×50). Haversian systems (arrowheads) and interstitial lamella (black asterisks) were observed, both of which are indicative of matured lamellar bone.

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