J Pathol Transl Med.  2016 Mar;50(2):104-112. 10.4132/jptm.2016.02.08.

Upregulated Neuro-oncological Ventral Antigen 1 (NOVA1) Expression Is Specific to Mature and Immature T- and NK-Cell Lymphomas

Affiliations
  • 1Department of Pathology, Yonsei University College of Medicine, Seoul, Korea. soyoon@yuhs.ac
  • 2Anatomic Pathology Reference Lab, Seegene Medical Foundation, Seoul, Korea.
  • 3Department of Internal Medicine, Division of Hematology, Yonsei University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
Recent studies have revealed that the splicing factor neuro-oncological ventral antigen 1 (NOVA1) is enriched in fibroblasts and accumulated T cells of tertiary lymphoid structures. In the present study, we investigated NOVA1 expression in various subtypes of mature and immature T- and natural killer (NK)-cell lymphomas as well as in various B-cell lymphoma subtypes.
METHODS
NOVA1 immunoexpression was evaluated in hyperplastic palatine tonsils (n = 20), T- and NK-cell lymphomas (n = 177), diffuse large B-cell lymphomas (n = 151), and other types of B cell lymphomas (n = 31). Nuclear staining intensity and percentage of positive tumor cells were graded. NOVA1 mRNA expression was analyzed in various lymphoma cell lines.
RESULTS
Tumor cells of T- and NK-cell lymphomas showed higher expression levels of NOVA1 than did normal paracortical T cells, and 56.5% of T- and NK-cell lymphoma cases showed diffuse and strong expression. The NOVA1 expression level varied according to the subtype; it was higher in angioimmunoblastic T-cell lymphoma, anaplastic lymphoma kinase (ALK)-negative anaplastic large cell lymphoma (ALCL), and T lymphoblastic leukemia/lymphoma (T-LBL), but it was lower in ALK-positive ALCL. In almost all B-cell lymphomas, NOVA1 expression was very low or negative. NOVA1 mRNA was also expressed in Jurkat, a T-LBL cell line.
CONCLUSIONS
The present findings suggest that NOVA1 upregulation may be involved in certain subtypes of T- and NK-cell lymphomas, but not in B-cell lymphomas. Upregulated NOVA1 expression seems to be a specific biological feature of activated T cells such as T- and NK-cell lymphomas.

Keyword

NOVA1; Lymphoma, T-cell; NK cell lymphoma; Splicing factor

MeSH Terms

Cell Line
Fibroblasts
Lymphoma*
Lymphoma, B-Cell
Lymphoma, Large-Cell, Anaplastic
Lymphoma, T-Cell
Palatine Tonsil
Phosphotransferases
RNA, Messenger
T-Lymphocytes
Up-Regulation
Phosphotransferases
RNA, Messenger
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