J Korean Ophthalmol Soc.  2004 Aug;45(8):1348-1355.

Neuroprotection Effect of Ascorbic Acid in Model of Ischemic Retinal Injury

Affiliations
  • 1Department of Ophthalmology, Gyeongsang National University College of Medicine, Chinju, Korea. stramast@gaechuk.gsnu.ac.kr

Abstract

PURPOSE
An attempt to establish the neuroprotection effect of ascorbic acid in ischemic retinal injury. METHODS: A rat anterior chamber was cannulated with a 30-gauge infusion needle connected to a container of balanced salt solution (BSS). The container was raised to a height of 3m during 60mins, thereby inducing retina ischemia by high intraocular pressure (HIOP). The effect of ascorbic acid was demonstrated by hematoxylin-eosin (H and E) stain and neuronal nitric oxide synthase (nNOS) immunohistochemical stain. The effect was classified into three HIOP-induced groups: without ascorbic acid treatment, ; ascorbic acid-treated at 24 hr and just before experiment; and ascorbic acid-treated at 24hr, just before experiment and every 24hr after experiment. RESULTS: After retinal ischemia, in the ascorbic acid-treatment groups (Ed- confirm the plural here; from above there are two ascorbic acid-treated groups), the thickness of each retinal layer was preserved more than that in the non-ascorbic acid treated group. There was little difference according to ascorbic acid administration time and period. By nNOS immunohistochemical stain, in the non-ascorbic acid administration group, nNOS immune reactive cells were increased remarkably, in the Inner Nuclear layer (INL) and Ganglion Cell Layer (GCL). In the ascorbic acid-treated groups (Ed-agin confirm), nNOS immune reactive cells were stained in a similar pattern to that of the control group. CONCLUSIONS: The neuroprotection effect of ascorbic acid in ischemic retinal injury was demonstrated, as was the suppression of nNOS expression in the ischemic retina tissue by ascorbic acid administration.

Keyword

Ascorbic acid; Ischemic retinal injury; nNOS; Neuroprotection

MeSH Terms

Animals
Anterior Chamber
Ascorbic Acid*
Ganglion Cysts
Intraocular Pressure
Ischemia
Needles
Nitric Oxide Synthase Type I
Rats
Retina
Retinaldehyde*
Ascorbic Acid
Nitric Oxide Synthase Type I
Retinaldehyde
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