J Korean Ophthalmol Soc.  2002 May;43(5):872-882.

The Effect of the Remnants after Cataract Extraction on the Lens Epithelial Cell Culture

• Do SJ
• Kang SJ
• Lee JH
• Oh JH
• Lee JH

Abstract

PURPOSE: To assess the effect of the remnants after lens extraction on posterior capsular opacification with lens epithelial cell culture through in vitro capsular bag model.
METHODS
After isolating porcine lens capsules, sterile non-toxic PMMA (polymethyl- mathacrylate) tension ring was inserted into the capsule. These were placed in organ culture medium up to 6 weeks. The grade of cell coverage of the posterior lens capsule was recorded to check the proliferative activity.
RESULTS
In the process of cell culture, outgrowth of the epithelial cells was observed across the posterior capsule after a lag period. The rate of cell coverage was dependent upon the added factors. The proliferative activity was the greatest in the group where lens cortical and nuclear materials were added, and other groups showed no difference from a control group.
CONCLUSIONS
To reduce posterior capsular opacification, it is important that we should not leave the lens cortical material behind during cataract surgery.

Keyword

In vitro capsular bag model; Lens epithelial cell; Posterior capsular opacity

MeSH Terms

Capsules
Cataract Extraction*
Cataract*
Cell Culture Techniques
Epithelial Cells*
Organ Culture Techniques
Polymethyl Methacrylate
Capsules
Polymethyl Methacrylate