J Korean Ophthalmol Soc.  2001 Apr;42(4):630-637.

The Effect of Lens Nucleus and Cortex Material on Lens Epithelial Cell Culture through In vitro Capsular Bag Model

• Lee J
• Chin H
• Oh J

Abstract

PURPOSE: This study attempts to evaluate the effect of lens cortex and nucleus remnants on posterior capsular opacification with method of the cell culture according to in vitro capsular bag model.
METHODS
After bovine lens were isolated, we performed continuous curvilinear capsulorhexis and hydrodissectioin of the lens fiber mass. At this stage a tension ring was implanted and then the preparations placed in organ culture for up to 6 weeks. Lens cortex and nucleus material was added at the culture media in group 2, 3, 4, 5, 6 with amount of 1/16, 1/32, 1/64, 1/96, 1/128 of one lens volume. Group 1 was control group that was not added lens materials. Cell coverage of the posterior lens capsule was recorded and the capsules were examined, both pre-and post-coverage, for proliferative activity.
RESULTS
After a lag period outgrowth was observed across the posterior capsule. The proliferative activity was greater at the groups that were added more amount of the lens cortex and nucleus material.
CONCLUSIONS
it is important that we should not remain any lens cortex material remnant at cataract surgery.

Keyword

Aftercataract; In vitro capsular bag model; Lens epithelial cell culture; Lens fiber

MeSH Terms

Capsules
Capsulorhexis
Cataract
Cell Culture Techniques
Culture Media
Epithelial Cells*
Organ Culture Techniques
Capsules
Culture Media