J Korean Orthop Res Soc.  2011 Jun;14(1):24-32.

Effect of a Long-term, Oral Fixed Dose with a Combination of Aledronate and Cacitriol on the Cancellous Bone Microarchitecture in Ovariectomized Rats

• Ji HM
• Nair SG
• Won YY
• Kim J
• Son MA

Abstract

PURPOSE
To study the effect of a long-term, oral, fixed dose with a combination of alendronate and calcitriol on the cancellous bone microarchitecture in an ovariectomized rat model.
MATERIALS AND METHODS
Twenty eight female Sprague-Dawley rats were divided into 2 equal groups: a non-medication group (OVX), and a medication group (ALD). The ALD group was treated with an oral daily fixed dose with a combination of alendronate and calcitriol for six months, starting from 4 weeks after ovariectomy, while the OVX group was given only a placebo. After six months, all animals were sacrificed, and an in vitro micro-CT analysis of the the distal femur was performed. The bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), trabecular number (Tb.N), structure model index (SMI), connectivity density (Conn.D), and bone mineral density (BMD) were assessed.
RESULTS
The ALD group had significantly higher BV/TV, Tb N, BMD and Conn.D and it also had significantly lower Tb Sp and SMI than the OVX group.
CONCLUSION
A long term, daily, oral fixed dose with a combination of alendronate and calcitriol could significantly reduce the osteoporotic changes in this ovariectomized rat model.

Keyword

Microarchitecture; Cancellous bone; Ovariectomized rat; Alendronate; Calcitriol; Long-term; Oral; Combination

MeSH Terms

Alendronate
Animals
Bone Density
Calcitriol
Female
Femur
Humans
Ovariectomy
Rats
Rats, Sprague-Dawley
Alendronate
Calcitriol