J Korean Acad Prosthodont.  2012 Oct;50(4):285-291. 10.4047/jkap.2012.50.4.285.

A study of mesenchymal stem cell proliferation and surface characteristics of the titanium discs coated with MS275/PLGA by an electrospray

• Yoo SY
• Kim SK
• Heo SJ
• Koak JY
• Lee JH
• Park YK
• Kim E

• KMID: 2195470
• DOI: http://doi.org/10.4047/jkap.2012.50.4.285

Abstract

PURPOSE
This study was conducted to identify the surface characteristics of titanium discs coated with MS275/PLGA by electrospray and which is effective to mesenchymal stem cell proliferation.
MATERIALS AND METHODS
We used anodized surface coated with PLGA as a control group and anodized surface coated with MS275 0.5 microM, 1 microM, 1.5 microM as test groups. To examine that the coating particles are nanometer sized, FE-SEM was used and AFM was utilized to determine the difference of coating surface roughness. We checked the mesenchymal stem cell proliferation by using MTT assay on 1st, 4th, 7th days.
RESULTS
There was no significant difference between control groups and test groups in AFM results (P>.05). In MTT assay results, mesenchymal stem cell proliferation was increased with time, at 7th day, cell viability on discs coated with 1.5 microM MS275 was significantly higher than control group (P<.05). As SEM showed, the number of cells on all discs was increased and the morphology of cell attachment was also wider and closer with time.
CONCLUSION
Titanium surface coated with MS275/PLGA showed significantly higher cell proliferation and the more density of MS275 was dispersed on titanium discs, the faster cells grew.

Keyword

Titanium; MS275; PLGA; Mesenchymal stem cells

MeSH Terms

Cell Proliferation
Cell Survival
Lactic Acid
Mesenchymal Stromal Cells
Polyglycolic Acid
Titanium
Lactic Acid
Polyglycolic Acid
Titanium

Figure

• 

  Fig. 1 SEM (×5000) image of anodized titanium disc by electrospraying (A) PLGA + DMSO (control)/disc. Anodized titanium discs are coated with submicron-sized PLGA particles. (B) 0.5 µM MS275 (25 µl) + PLGA (25 µl))/disc. Anodized titanium disc coated with PLGA/MS275 shows more sticky and fine texture than control group. (C) 1.0 µM MS275 (25 µl) + PLGA (25 µl))/disc (d) 1.5 µM MS275 (25 µl) + PLGA (25 µl))/disc.

• 

  Fig. 2 MTT assay measured as cell proliferation. The result shows that cell proliferation is increased by time and after 7 day of cell culture, there is a significant difference (*P<.05; the graph bar represents mean ± SE). And the cell activity of test groups (anodized titanium disc coated with PLGA + MS275 0.5 µM, 1.0 µM, 1.5 µM) is significantly higher than control group (anodized titanium disc coated with PLGA/DMSO) at 7 day.

• 

  Fig. 3 SEM (×500) images of 1 day cell culture (A) PLGA + DMSO (control) /disc, (B) 0.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, (C) 1.0 µM MS275 (25 µl) + PLGA (25 µl))/disc, (D) 1.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, SEM (×500) images of 4 day cell culture, (E) PLGA + DMSO (control) /disc, (F) 0.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, (G) 1.0 µM MS275 (25 µl) + PLGA (25 µl))/disc, (H) 1.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, SEM (×500) images of 7 day cell culture, (I) PLGA + DMSO (control)/disc, (J) 0.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, (K) 1.0 µM MS275 (25 µl) + PLGA (25 µl))/disc, (L) 1.5 µM MS275 (25 µl) + PLGA (25 µl))/disc.

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  Fig. 4 The results of surface roughness measured by AFM (20 µm × 20 µm) (A) Anodized titanium disc coated with PLGA, (B) Anodized titanium disc coated with MS275 and PLGA. There is no significant difference between group (A) and (B) (P>.05).

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