J Korean Orthop Assoc.  2010 Feb;45(1):44-51. 10.4055/jkoa.2010.45.1.44.

Comparison of the Nerve Regenerating Effect of Inside-out Vein Grafts, Muscle-filled Inside-out Vein Grafts, and Fat-interposed Inside-out Vein Grafts in Rats

• Lee JH
• Chung DW

• KMID: 2185593
• DOI: http://doi.org/10.4055/jkoa.2010.45.1.44

Abstract

PURPOSE
We wanted to compare the nerve regeneration of inside-out vein grafts, muscle-filled inside-out vein grafts and fat-interposed inside-out vein grafts to repair the 10 mm sciatic nerve defects in rats.
MATERIALS AND METHODS
We performed inside-out vein grafts, muscle-filled inside-out vein grafts and fat-interposed inside-out vein grafts to repair the 10 mm sciatic nerve defects in 45 rats. Histological examinations (the numbers and diameters of myelinated nerve fibers), electrophysiologic testing (Compound Muscle Action Potential (CMAP) and Nerve Conduction Velocity (NCV)) examinations were performed 3 months after surgery.
RESULTS
Quantitative analysis of myelinated nerve fibers showed that total number of nerve fibers was significantly greater in group 1 or 3 than that in group 2. There were statistical differences in the three groups for the fiber diameters of the myelinated fibers. Electrophysiologic examination showed that both CMAP and NCV were significantly greater in group 3 than that in group 2 and there was no statistical difference between in groups 1 and 3.
CONCLUSION
This study shows that fat-interposed inside-out vein grafts have excellent axonal regeneration as compared to that of inside-out vein grafts or muscle-filled inside-out vein grafts to repair 10 mm sciatic nerve defects in rat. Therefore fat-interposed inside-out vein grafts are an effective method to accelerate the early axonal regeneration for repairing nerve defects.

Keyword

rat sciatic nerve; nerve regeneration; inside-out vein graft; muscle; fat

MeSH Terms

Action Potentials
Animals
Axons
Muscles
Myelin Sheath
Nerve Fibers
Nerve Fibers, Myelinated
Nerve Regeneration
Neural Conduction
Rats
Regeneration
Sciatic Nerve
Transplants
Veins

Figure

• 

  Figure 1 Rat sciatic nerve repaired by means of inside out vein graft alone, group 1 (Masson's Trichrome stain, (A) ×400, (B) ×1000). Photomicrograph of a semithin cross-section shows a high number and small size of axon.

• 

  Figure 2 Rat sciatic nerve repaired by means of muscle-filled inside-out vein graft, group 2 (Masson's Trichrome stain, (A) ×400, (B) ×1000). Photomicrograph of a semithin cross-section shows increased fibrosis between the regenerated axon and small number of axon.

• 

  Figure 3 Rat sciatic nerve repaired by means of fat-interposed inside-out vein graft, group 3 (Masson's Trichrome stain, (A) ×400, (B) ×1000). This group shows more regular shape of axon and more parallel and organized regeneration.

• 

  Figure 4 EMG records at 3 months postoperatively. (A) Inside-out vein graft alone. (B) Muscle-filled inside out vein graft. (C) Fat-interposed inside-out vein graft.

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