J Korean Acad Conserv Dent.  2007 Mar;32(2):102-110. 10.5395/JKACD.2007.32.2.102.

Detection of methicillin or vancomycin-resistant Staphylococcus aureus from dental hospital

• Min JH
• Park SN
• Hwang HK
• Min JB
• Kim HS
• Kook JK

• KMID: 2175843
• DOI: http://doi.org/10.5395/JKACD.2007.32.2.102

Abstract

The purpose of this study was to obtain the basic information for the improvement of dental environment by investigating the presence of methicillin- or vancomycin-resistant Staphylococcus aureus (MRSA or VRSA) isolated from dental health care workers (DHCWs) and environment of the Chosun University Dental Hospital (CUDH) and a private dental clinic (control group). Staphylococcus aureus (S. aureus) was isolated from anterior nares of 42 DHCWs and 38 sites, unit chairs, x-ray devices, computers, etc., at 10 departments of the CUDH and 20 DHCWs and 11 sites at the private dental clinic. S. aureus was isolated on mannitol salt agar plate and confirmed by PCR with S. aureus species-specific primer. Antimicrobial susceptibility test of clinical isolates of S. aureus against several antibiotics including methicillin (oxacillin) was performed by investigating minimum inhibitory concentration (MIC) using broth microdilution assay. In addition, PCR was performed to detect the methicillin- or vancomycin-resistant gene. The data showed that one strain of S. aureus was isolated from DHCWs of the CUDH and three strains of S. aureus was isolated from 3 samples of the private dental clinic, respectively. All of the isolates from the CUDH and the private dental clinic had resistance to penicillin G, amoxicillin and vancomycin and susceptibility to oxacillin and ciprofloxacin. The S. aureus strains were already obtained the resistance to penicillin G and amoxicillin. These results suggest that two dental clinics were under relatively safe environment.

Keyword

MRSA; VRSA; Minimum inhibitory concentration; PCR

MeSH Terms

Agar
Amoxicillin
Anti-Bacterial Agents
Ciprofloxacin
Delivery of Health Care
Dental Clinics
Mannitol
Methicillin*
Methicillin-Resistant Staphylococcus aureus
Microbial Sensitivity Tests
Oxacillin
Penicillin G
Polymerase Chain Reaction
Staphylococcus aureus*
Staphylococcus*
Vancomycin
Agar
Amoxicillin
Anti-Bacterial Agents
Ciprofloxacin
Mannitol
Methicillin
Oxacillin
Penicillin G
Vancomycin

Figure

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  Figure 1 Isolation of S. aureus on mannitol salt agar (MSA) which is selective medium for the S. aureus. The color change of the medium to yellow means that mannitol was fermented by S. aureus (A). S. epidermidis had no ability of the fermentation of mannitol (B).

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