Chonnam Med J.
2001 Mar;37(1):7-12.
Nuclear Factors Bind to the Specific Sites in Promoter Region of Human YB-1 Gene
- Affiliations
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- 1Research Institute of Medical Sciences, Chonnam National University, Kwangju, Korea.
Abstract
- This study was designed to identify the presence of the nuclear factors binding to promoter region of human YB-1 gene and determine their specific binding sites. Nuclear extracts were prepared from Chang liver, Hep G2 and CT-26 cells and electrophoretic mobility shift assay was performed to determine the specific binding sites. The nuclear factors isolated from three cell lines commonly bind to the C8 (+96~+125) site in promoter region of YB-1 gene. The nuclear factors prefer binding to double-stranded DNA rather than binding to single-stranded DNA. These data suggest that C8 is the important binding site for the nuclear factors presumably regulating the transcription of human YB-1 gene.
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