Chonnam Med J.  2014 Aug;50(2):58-62. 10.4068/cmj.2014.50.2.58.

Levels of Salivary Antioxidant Vitamins and Lipid Peroxidation in Patients with Oral Lichen Planus and Healthy Individuals

• Abdolsamadi H
• Rafieian N
• Goodarzi M
• Feradmal J
• Davoodi P
• Jazayeri M
• Taghavi Z
• Hoseyni SM
• Ahmadi-Motamayel F

• KMID: 2172160
• DOI: http://doi.org/10.4068/cmj.2014.50.2.58

Abstract

Oral lichen planus (OLP) is a chronic inflammatory mucosal disease of unknown etiology. Many studies have implicated the protective role of antioxidants in such diseases. The aim of this study was to compare salivary total antioxidant capacity (TAC and malondialdehyde (MDA) and antioxidant vitamin (vitamin s A, C and E) levels in patients with erosive OLP and healthy individuals. Thirty six patients with OLP (14 males, 22 females) and 36 control subjects (15 males, 21 females), matched for age and sex were enrolled in this case control study. The salivary levels of MDA, TAC, and antioxidant vitamin levels were measured in both case and control groups. The salivary level of MDA was significantly higher (p<0.001) in patients than in controls. In patients with OLP, the TAC of saliva was significantly lower than that in healthy subjects (p<0.001). Compared with controls, the levels of salivary antioxidant vitamins were significantly decreased in patients with OLP (p<0.001). In addition, a positive correlation was found between the decrease in the salivary amount of vitamin C and that in vitamin E in patients and controls. In addition to the lower salivary levels of antioxidant vitamins and the lower TAC, the higher level of MDA in patients with OLP suggests that free radicals and the resulting oxidative damage may be important in the pathogenesis of OLP lesions.

Keyword

Oral Lichen Planus; Antioxidants; Oxidative Stress; Saliva

MeSH Terms

Antioxidants
Ascorbic Acid
Case-Control Studies
Free Radicals
Humans
Lichen Planus, Oral*
Lipid Peroxidation*
Male
Malondialdehyde
Oxidative Stress
Saliva
Vitamin E
Vitamins*
Antioxidants
Ascorbic Acid
Free Radicals
Malondialdehyde
Vitamin E
Vitamins

Figure

• 

  FIG. 1 The unstimulated whole saliva concentrations of (A) malondialdehyde (MDA) and (B) total antioxidant activity (TAC) in cases (patients with erosive oral lichen planus) and controls (healthy subjects). *Indicates significant difference with control (p<0.001).

• 

  FIG. 2 Positive correlation between salivary levels of vitamin C and vitamin E in patients and controls. r=0.374 and p value=0.001.

Reference

1. Porter SR, Kirby A, Olsen I, Barrett W. Immunologic aspects of dermal and oral lichen planus: a review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997; 83:358–366.

2. Walsh LJ, Savage NW, Ishii T, Seymour GJ. Immunopathogenesis of oral lichen planus. J Oral Pathol Med. 1990; 19:389–396.

3. Thongprasom K, Chaimusig M, Korkij W, Sererat T, Luangjarmekorn L, Rojwattanasirivej S. A randomized-controlled trial to compare topical cyclosporin with triamcinolone acetonide for the treatment of oral lichen planus. J Oral Pathol Med. 2007; 36:142–146.

4. Roopashree MR, Gondhalekar RV, Shashikanth MC, George J, Thippeswamy SH, Shukla A. Pathogenesis of oral lichen planus--a review. J Oral Pathol Med. 2010; 39:729–734.

5. Chainani-Wu N, Silverman S Jr, Lozada-Nur F, Mayer P, Watson JJ. Oral lichen planus: patient profile, disease progression and treatment responses. J Am Dent Assoc. 2001; 132:901–909.

6. Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem. 1997; 43:1209–1214.

7. Nagao T, Warnakulasuriya S, Ikeda N, Fukano H, Yamamoto S, Yano M, et al. Serum antioxidant micronutrient levels in oral lichen planus. J Oral Pathol Med. 2001; 30:264–267.

8. Alfadda AA, Sallam RM. Reactive oxygen species in health and disease. J Biomed Biotechnol. 2012; 2012:936486.

9. Scully C, Beyli M, Ferreiro MC, Ficarra G, Gill Y, Griffiths M, et al. Update on oral lichen planus: etiopathogenesis and management. Crit Rev Oral Biol Med. 1998; 9:86–122.

10. Keller KL, Fenske NA. Uses of vitamins A, C, and E and related compounds in dermatology: a review. J Am Acad Dermatol. 1998; 39:611–625.

11. Yang LL, Liu XQ, Liu W, Cheng B, Li MT. Comparative analysis of whole saliva proteomes for the screening of biomarkers for oral lichen planus. Inflamm Res. 2006; 55:405–407.

12. Sezer E, Ozugurlu F, Ozyurt H, Sahin S, Etikan I. Lipid peroxidation and antioxidant status in lichen planus. Clin Exp Dermatol. 2007; 32:430–434.

13. Azizi A, Farshchi F. Comparison of salivary and plasma antioxidant levels in lichen planus patients and healthy subjects. J Oral Pathol Med. 2012; 41:524–526.

14. Al-Hashimi I, Schifter M, Lockhart PB, Wray D, Brennan M, Migliorati CA, et al. Oral lichen planus and oral lichenoid lesions: diagnostic and therapeutic considerations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007; 103:Suppl. S25.e1–S25.e12.

15. Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. 1999; 299:15–27.

16. Esterbauer H, Zollner H. Methods for determination of aldehydic lipid peroxidation products. Free Radic Biol Med. 1989; 7:197–203.

17. Roe JH, Kuether CA. The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid. J Biol Chem. 1943; 147:399–407.

18. Aly DG, Shahin RS. Oxidative stress in lichen planus. Acta Dermatovenerol Alp Pannonica Adriat. 2010; 19:3–11.

19. Mohit B, Shelja V, Nidhi G, Sunint S. Antioxidants - Its Preventive Role In Oral Cancer. Indian J Dent Sci. 2012; 4:103–105.

20. Jacob RA, Burri BJ. Oxidative damage and defense. Am J Clin Nutr. 1996; 63:985S–990S.

21. Barikbin B, Yousefi M, Rahimi H, Hedayati M, Razavi SM, Lotfi S. Antioxidant status in patients with lichen planus. Clin Exp Dermatol. 2011; 36:851–854.

22. Chan AC. Partners in defense, vitamin E and vitamin C. Can J Physiol Pharmacol. 1993; 71:725–731.

23. Agha-Hosseini F, Mirzaii-Dizgah I, Mikaili S, Abdollahi M. Increased salivary lipid peroxidation in human subjects with oral lichen planus. Int J Dent Hyg. 2009; 7:246–250.

24. Hassan I, Keen A, Majid S, Hassan T. Evaluation of the antioxidant status in patients of lichen planus in Kashmir valley - A hospital based study Iffat Hassan. J Saudi Soc Dermatol Dermatol Surg. 2013; 17:13–16.

25. Ergun S, Troşala SC, Warnakulasuriya S, Özel S, Önal AE, Ofluoğlu D, et al. Evaluation of oxidative stress and antioxidant profile in patients with oral lichen planus. J Oral Pathol Med. 2011; 40:286–293.

26. Bureau I, Gueux E, Mazur A, Rock E, Roussel AM, Rayssiguier Y. Female rats are protected against oxidative stress during copper deficiency. J Am Coll Nutr. 2003; 22:239–246.